Overview
Type: Synthetic peptide
Form: Lyophilized powder
Homer1 Blocking Peptide (#BLP-PZ026) is the original antigen used for immunization during Anti-Homer1 Antibody (#APZ-026) generation. The blocking peptide binds and ‘blocks’ Anti-Homer1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain synaptosomal fractions (lanes 2 and 4):1,2. Anti-Homer1 Antibody (#APZ-026), (1:500).
3,4. Anti-Homer1 Antibody, preincubated with Homer1 Blocking Peptide (#BLP-PZ026).
Expression of Homer1 in mouse hippocampus and striatumImmunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-Homer1 Antibody (#APZ-026), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. A. Homer1 staining (green) in hippocampal dentate gyrus region, is detected in neuronal soma (arrows). B. Homer1 staining in the striatum region, also shows labeling in neuronal soma (arrows). Cell nuclei are stained with DAPI (blue).
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain synaptosome lysates (lanes 2 and 4):1-2. Guinea Pig Anti-Homer1 Antibody (#APZ-026-GP), (1:400).
3-4. Guinea Pig Anti-Homer1 Antibody, preincubated with Homer1 Blocking Peptide (BLP-PZ026).
Expression of Homer1 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea Pig Anti-Homer1 Antibody (APZ-026-GP). Sections were incubated with guinea pig Anti-Homer1 (APZ-026-GP), (1:100), followed by goat Anti guinea pig conjugated with Alexa 594 (red). A, staining appeared in the soma and apical dendrites of neurons (arrows). B, Pre-incubation of the antibody with Homer1 blocking peptide (BLP-PZ026), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1 (Accession Q9Z214).
Accession (Uniprot) Number Q9Z214
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
