Recombinant human BDNF proDomain protein

Human Brain-Derived Neurotrophic Factor proDomain, Recombinant, E. coli
    Cat #: B-245
  • Lyophilized Powder
  • Bioassay Tested
  • Sterile & Endotoxin Free
  • Origin Recombinant, E. coli
    MW: 12.4 kDa.
    Endotoxin Level <0.1 EU per 1 µg of the protein by the LAL method.
    Purity: >98% (HPLC)
    Form Lyophilized from a 0.2 µm filtered solution.
    • Recombinant human BDNF proDomain protein
    Activity BDNF is a neurotrophic factor and binds p75NTR as well as TrkB receptors1,2. BDNF supports the survival of many cell types3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons9 expressing both p75 and sortilin, and to be involved in LTD10.
    1. Tolkovsky, A. (1997) Trends Neurosci20, 1.
    2. Jing, S. et al. (1992) Neuron 9, 1067.
    3. Acheson, A. et al. (1995) Nature 374, 450.
    4. Morse, J.K. et al. (1993) J. Neurosci13, 4146.
    5. Hyman, C. et al. (1991) Nature 350, 230.
    6. Friedman, B. et al. (1995) J. Neurosci15, 1044.
    7. Meyer, M. et al. (1992) J. Cell Biol119, 45.
    8. Koliatsos, V.E. et al. (1993) Neuron 10, 359.
    9. Teng, H.K. et al. (2005) J. Neurosci25, 5455.
    10. Woo, N.H. et al. (2005) Nat. Neurosci8, 1069.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Sterile water at a concentration of at least 10 µg/ml.
    Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
    Storage of solutions Up to one week at 4°C or four-six weeks at -70°C.
    Our bioassay
    References - Scientific background
    1. Segal, R. (2001) Sci. STKE. 2001, pe1.
    2. Hariri, A.R. et al. (2003) J. Neurosci23, 6690.
    3. Fahnestock, M. et al. (2001) Mol. Cell. Neurosci. 18, 210.
    4. Dechant, G. et al. (1993) Development 119, 545.
    5. Mowla, S.J. et al. (2001) J. Biol. Chem276, 12660.
    6. Teng, H.K. et al. (2005) J. Neurosci25, 5455.
    7. Gray, A.M. and Mason, A.J. (1990) Science 247, 1328.
    8. Gregory, K.E. et al. (2005) J. Biol. Chem280, 27970.
    9. Egan, M. et al. (2003) Cell 112, 257.
    10. Quistgaard, E.M. et al. (2008) Nat. Struct. Mol. Biol. 16, 96.
    Scientific background

    BDNF regulates neuronal survival, differentiation, and synaptic plasticity. It affects the release of excitatory neurotransmitters and has been found to affect cardiovascular development and function.1 Like many other neurotrophins, BDNF is a cleavage product of the BDNF precursor, proBDNF. This precursor may be cleaved by various proteases, intracellularly by furin and extracellularly by several proteases including prohormone convertases, plasminogen activator, MMP-3 and MMP-7 in vitro.2,3

    Two different trans-membrane receptor proteins mediate BDNF and proBDNF signal transduction: the TrkB, and the pan-neurotrophic receptor p75NTR.4 ProBDNF has been demonstrated to induce TrkB phosphorylation in vitro and to bind p75NTR and sortilin to promote apoptosis.5,6

    In many cases, the full prodomain region derived from the protein precursor has biological functions, for instance; the prodomain of the transforming growth factor β (TGFβ) affects the dimerization and folding as well as the activity of the mature proteins via non-covalent association. The propeptide of the bone morphogenetic proteins BMP-4 and BMP-7 regulates the diffusion and distribution of these growth factors within the extracellular matrix.7,8 The prodomain region of the BDNF precursor plays an important role in regulating its intracellular trafficking to secretory pathways.9 However, the role of the full BDNF-prodomain, which is a product of proteolytic cleavage of proBDNF, is not clearly understood. Furthermore, binding competition studies suggest that binding sites for BDNF prodomain are located in the tunnel of the ten-bladed b-propeller domain of sortilin.10

    Net Peptide Content: 100%
    Last update: 24/01/2020

    Recombinant human BDNF proDomain protein (#B-245) is a highly pure, recombinant, and biologically active protein.

    For research purposes only, not for human use
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