Human CysLTR2 (extracellular) Blocking Peptide (#BLP-LR004) is the original antigen used for immunization during Anti-Human CysLTR2 (extracellular) Antibody (#ALR-004) generation. The blocking peptide binds and ‘blocks’ Anti-Human CysLTR2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of acute monocytic leukemia THP-1 (lanes 1 and 4), promyelocytic leukemia HL-60 (lanes 2 and 5) and chronic myelogenous leukemia K562 (lanes 3 and 6) human cell lysates:
- (C)HLTTWKVGL(S)KDRLH, corresponding to amino acid residues 270-284 of human CysLTR2 (Accession Q9NS75).