Human GFRα1-Fc Chimera

An Inhibitor of GDNF Activity
    Cat #: RPC-003
  • Lyophilized Powder
  • Bioassay Tested
  • Sterile & Endotoxin Free
  • Origin Recombinant, HEK 293-6E cells.
    MW: ~ 85 kDa.
    Endotoxin Level <0.1 EU per 1 µg of the protein by the LAL method.
    Purity: >90% (HPLC)
    Form The product is lyophilized from a 0.2 µm filtered solution in PBS.
    Effective concentration 4-10 µg/ml in the presence of 10 ng/ml of human GDNF.
    Sequence Human GFRα1 (Ala19-Lys429)-IEGRMD-Human IgG1 (Pro100-Lys330).
    Modifications Glycosylation.
    Activity Human GFRα1-Fc Chimera inhibits GDNF-induced phosphorylation of ERK1/2 MAPK and Akt in SH-5Y5Y cells.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Water. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
      • Human GFRα1-Fc Chimera
        Alomone Labs Human GFRα1-Fc Chimera inhibits GDNF-induced ERK1/2 MAPK and Akt phosphorylation in SH-SY5Y cells.
        Cells were serum deprived for 2 hr and then stimulated for 10 min with 10 ng/ml Recombinant human GDNF protein (#G-240) (lane 2), 4 µg/ml Human GFRα1-Fc Chimera (#RPC-003) preincubated with 10 ng/ml  Recombinant human GDNF protein for 90 min (lane 3). Control cells incubated without Recombinant human GDNF protein are shown in lane 1. Cell proteins were resolved by SDS-PAGE and detected with anti-phospho-Akt (A) or with anti-ERK1/2 MAPK (B).
    References - Scientific background
    • 1. Lin, L.F. et al. (1993) Science 260, 1130.
    • 2. Treanor, J.J. et al (1996) Nature 382, 80.
    • 3. Jing, S. et al. (1997) J. Biol. Chem. 272, 33111.
    • 4. Angrist, M. et al. (1998) Genomics 48, 354.
      • GDNF (Glial cell line neurotrophic factor) is a neurotrophic factor that increases the survival of midbrain dopaminergic neurons and is essential for the development of the kidneys and the enteric nervous system. This factor is a glycosylated disulfide-bonded homodimer and is distantly related to the growth factor-β super family1.

        GDNF binds with high affinity in GDNF responsive cells to the Glycosyl-phosphatidylinositol (GPI) linked receptor known as GDNFR or GFRα12 and also requires the presence of the Ret protein tyrosine kinase for the continuation of signal transduction3.

        GFRα1 is an anchored cell surface molecule and was mapped to the human chromosome 10. It was found to have an integral role in RET proto oncogene-mediated signaling thus being linked to disorders such as Hirschsprung’s disease in case of a loss of function mutation and various cancers in gain of function mutations4.

        Human GFRα1-Fc Chimera inhibits GDNF-induced phosphorylation of ERK1/2 MAPK and Akt in SH-5Y5Y cells and can prove as an important ligand in the research of GDNF associated diseases.

    Target GDNF
    Net Peptide Content: 100%
    Last update: 06/11/2019

    Human GFRα1-Fc Chimera (#RPC-003) is a highly pure, recombinant, and biologically active protein.

    For research purposes only, not for human use