Human GLUT3 (extracellular) Blocking Peptide (#BLP-GT043) is the original antigen used for immunization during Anti-Human GLUT3 (extracellular) Antibody (#AGT-043) generation. The blocking peptide binds and ‘blocks’ Anti-Human GLUT3 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 6), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 7), human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 8), human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 4 and 9) and human Jurkat T-cell leukemia cell line lysate (lanes 5 and 10):
- (C)KEFINKTLTDKGN, corresponding to amino acid residues 39 - 51 of human GLUT3 (Accession P11169).