Human GPR132 (extracellular) Blocking Peptide (#BLP-GR046) is the original antigen used for immunization during Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046) generation. The blocking peptide binds and ‘blocks’ Anti-Human GPR132 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human Raji B lymphocyte lymphoma cell line lysate (lanes 2 and 5) and human HL-60 promyelocytic leukemia cell line lysate (lanes 3 and 6):
- (C)EDKET(S)FDMLQMDSR, corresponding to amino acid residues 181 - 195 of human GPR132 (Accession Q9UNW8).