Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human GPR132 (extracellular) Blocking Peptide (#BLP-GR046) is the original antigen used for immunization during Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046) generation. The blocking peptide binds and ‘blocks’ Anti-Human GPR132 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 4), human Raji B lymphocyte lymphoma cell line lysate (lanes 2 and 5) and human HL-60 promyelocytic leukemia cell line lysate (lanes 3 and 6):1-3. Anti-Human GPR132/G2A (extracellular) Antibody (#AGR-046), (1:200).
4-6. Anti-Human GPR132/G2A (extracellular) Antibody, preincubated with Human GPR132 (extracellular) Blocking Peptide (#BLP-GR046).
Properties
Sequence
- (C)EDKET(S)FDMLQMDSR, corresponding to amino acid residues 181 - 195 of human GPR132 (Accession Q9UNW8).
Accession (Uniprot) Number Q9UNW8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
