Overview
Type: Synthetic peptide
Form: Lyophilized powder
Human TRPM1 Blocking Peptide (#BLP-CC041) is the original antigen used for immunization during Anti-Human TRPM1 Antibody (#ACC-041) generation. The blocking peptide binds and ‘blocks’ Anti-Human TRPM1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of A875 (human melanoma cell line) lysate:1. Anti-Human TRPM1 Antibody (#ACC-041), (1:200).
2. Anti-Human TRPM1 Antibody, preincubated with Human TRPM1 Blocking Peptide (#BLP-CC041).
Properties
Sequence
- (C)EEKKVKKEKASTETE, corresponding to amino acid residues 1518-1533 of human TRPM1 (Accession O75560).
Accession (Uniprot) Number O75560
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
