Overview
Type: Synthetic peptide
Form: Lyophilized powder
HVCN1 Blocking Peptide (#BLP-HC001) is the original antigen used for immunization during Anti-HVCN1 Antibody (#AHC-001) generation. The blocking peptide binds and ‘blocks’ Anti-HVCN1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse WEHI B-cell lymphoma (lanes 1 and 4), human HL-60 promyelocytic leukemia (lanes 2 and 5) and human THP-1 acute monocytic leukemia (lanes 3 and 6) cell lysates:1-3. Anti-HVCN1 Antibody (#AHC-001), (1:200).
4-6. Anti-HVCN1 Antibody, preincubated with HVCN1 Blocking Peptide (#BLP-HC001).
Properties
Sequence
- (C)GDDYHTWNVNYKK, corresponding to amino acid residues 32-44 of mouse HVCN1 (Accession Q3U2S8).
Accession (Uniprot) Number Q3U2S8
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
