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Certificate of Analysis

Intracellular Ion Channel Antibody Explorer Kit

A Screening Package of Intracellular Ion Channel Antibodies Economically Priced

Cat #: AK-233
Size: 12 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-CALHM1 Antibody Cat #: ACC-101

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EAMNHDLELGHTHG, corresponding to amino acid residues 252-265 of human CALHM1 (Accession Q8IU99). Intracellular, C-terminus.
Homology
Rat, mouse - identical.

Anti-CLIC1 Antibody Cat #: ACL-021

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, WB Reactivity: H, M, R
Immunogen
Peptide (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1 (Accession O00299). Intracellular, C-terminus.
Homology
Rat, mouse - identical.

Anti-CLIC4 Antibody Cat #: ACL-024

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, WB Reactivity: H, M, R
Immunogen
Peptide (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4 (Accession Q9Y696). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-CLIC5 Antibody Cat #: ACL-025

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161-174 of rat CLIC5 (Accession Q9EPT8). Intracellular, C-terminus.
Homology
Mouse - identical; human - 9/14 amino acid residues identical.

Anti-IP3 Receptor-1 (ITPR1) Antibody Cat #: ACC-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RIGLLGHPPHMNVNPQQPA, corresponding to amino acid residues 2732-2750 of rat IP3R1 (Accession P29994). Intracellular, C-terminus.
Homology
Human, mouse - identical.

Anti-IP3 Receptor-2 (ITPR2) Antibody Cat #: ACC-116

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: R
Immunogen
Peptide (C)RLGFLGSNTPHENH, corresponding to amino acid residues 2683-2696 of rat IP3R2 (Accession P29995). Intracellular, C-terminus.
Homology
Mouse, human - 13/14 amino acid residues identical.

Anti-Ryanodine Receptor 1 Antibody Cat #: ARR-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)RAENEKDATTEKNKKR, corresponding to amino acid residues 1371-1386 of human RyR1 (Accession P21817). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-Ryanodine Receptor 2 Antibody Cat #: ARR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human RyR2 (Accession Q92736). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-Ryanodine Receptor 3 Antibody Cat #: ARR-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human RyR3 (Accession Q15413). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

Anti-TRIC-A (TMEM38A) Antibody Cat #: ATC-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IH, WB Reactivity: M, R
Immunogen
Peptide (C)DNHGAPHGMGLGTQHS, corresponding to amino acid residues 259-274 of rat TRIC-A (Accession A6ZIQ8). Intracellular, C-terminus.
Homology
Mouse - identical; human - will not recognize human TRIC-A.

Anti-TRIC-B (TMEM38B) Antibody Cat #: ATC-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)GMKEVTRTWKIVG, corresponding to amino acid residues 116-126 of rat TRIC-B (Accession Q68FV1). Intracellular, cytoplasmic loop.
Homology
Mouse, human - identical.

Anti-VDAC Antibody Cat #: AVC-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)DGKNVNAGGHK, corresponding to amino acid residues of 264-274 of rat VDAC1 (Accession Q9Z2L0). Intracellular.
Homology
VDAC1: rat, mouse, human identical; VDAC2: rat, mouse, human - 9/11 amino acid residues identical; VDAC3: rat, mouse - 10/11 amino acid residues identical, human - 9/11 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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