IRL-1620

Suc-[Glu9, Ala11,15] Endothelin-1, (8-21)
A Selective and Potent Endothelin B Receptor Agonist
Cat #: SPE-220
Alternative Name Suc-[Glu9, Ala11,15] Endothelin-1, (8-21)
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1820 Da.
    Purity: ≥98% (HPLC)
    Effective concentration 1 pM -100 nM.
    Sequence DEEAVYFAHLDIIW.
    Modifications Asp1 = Suc-Asp.
    Structure
    • IRL-1620
    Molecular formula C86H117N17O27.
    CAS No.: 142569-99-1.
    Activity IRL-1620 is a selective and potent ET-B receptor agonist1,2.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Soluble in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • IRL-1620
      Alomone Labs IRL-1620 activates Endothelin B receptor-mediated Ca2+ mobilization in CHO-K1-mt Aequorin cells.
      Dose-response curve of normalized activation of Endothelin B receptor-mediated Ca2+ mobilization, evoked by IRL-1620 (#SPE-220). Ca2+ response was detected by measuring aequorin-derived fluorescence following application of IRL-1620 and Endothelin-1 at different concentrations. The EC50 measured for IRL-1620 is 1.53 nM.
    References - Scientific background
    1. Bell, K.M. et al. (1999) Int. J. Cancer 80, 295.
    2. Xie, C. and Wang, D.H. (2009) Hypertension 54, 1298.
    3. Davenport, A.P. et al. (2016) Pharmacol. Rev. 68, 357.
    Scientific background

    IRL-1620 is a synthetic peptide that acts as a selective and potent ET-B (endothelin B) receptor agonist, a receptor belonging to the G-protein coupled receptors (GPCR) superfamily. IRL-1620 is a C-terminal linear peptide analog of endothelin (ET-1) with a structure that is based on the carboxy-terminal end of ET-1. IRL-1620 has the ability to produce vasodilation or vasoconstriction1.

    Endothelin 1 (ET-1) and its receptor ET-B play an important role in regulating renal function and blood pressure. ET-B receptor and ET-1 are both expressed in sensory nerves. They are mainly present in medium- and large-sized cell bodies of human trigeminal ganglia2.

    Studies have shown that acute administration of an ET-B receptor agonist into the rat renal medulla leads to natriuresis and diuresis via a mechanism that is NOS1-dependent3.

    Target ET-B receptor
    Net Peptide Content: 100%
    Last update: 27/01/2020

    IRL-1620 (#SPE-220) is a highly pure, synthetic, and biologically active peptide.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Shipping and Ordering information