Isopimaric Acid

IPA
A Novel Opener of KCa1.1 Channels
    Cat #: I-370
    Alternative Name IPA
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Natural product of unspecified plant origin.
    Source Natural
    MW: 302
    Purity: >99%
    Effective concentration 1-100 μM.
      • Isopimaric Acid
    Chemical name 1-Phenanthrenecarboxylicacid,7-ethenyl- 1,2,3,4,4a,4b,5,6,7,8,10,10a-dodecahyde.
    Molecular formula C20H30O2.
    CAS No.: 5835-26-7.
    Activity Isopimaric acid is a potent opener of large conductance Ca2+-activated K+ (BK) channels. 1 μM of Isopimaric acid doubles the current carried via BK channels. Isopimaric acid may increase the open probability by shifting the Ca2+ and voltage dependence to more hyperpolarized voltages1. The sensitivity is decreased in the Xenopus system compared to mammalian cells. BK channels activated by Isopimaric acid retain their charybdotoxin sensitivity.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO, ethanol, acetone or methanol. DMSO is suggested for biological experiments. DMSO solution should not exceed 0.03%. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to three months at -20°C.
      • Isopimaric Acid
        Alomone Labs Isopimaric Acid was tested in Xenopus oocytes expressing mSlo (BK) channel using TEVC recordings.
        Time course of BK channel amplitudes, by applying 100 μM Isopimaric Acid (#I-370). Currents were elicited by a 150 ms pulse that was increased stepwise every 30 ms from +10 mV to +50 mV from a holding potential of -100 mV. Pulses were delivered every 10 sec. Pulses were normalized to the values of controls.
    References - Scientific background
      • Isopimaric acid (originally isolated from terpenoids) was found to be a potent opener of KCa1.1 (large conductance Ca2+-activated K+ (BK) channels. 1 µM Isopimaric acid doubled the current carried via KCa1.1 channels and this effect increased dose dependently. In analogy to the effects of pimaric acid, isopimaric acid may increase the open probability of single KCa1.1 channels by shifting both its voltage and Ca2+ dependence to more hyperpolarized voltages.1 KCa1.1 channels that were activated by isopimaric acid retain their charybdotoxin sensitivity.
    Target KCa1.1 K+ channels
    Last update: 13/11/2019

    Isopimaric Acid (#I-370) is a highly pure, natural, and biologically active compound.

    For research purposes only, not for human use