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Jingzhaotoxin-X

JZTX-X, Jingzhaotoxin F7-15.33

A Blocker of KV4.2 and KV4.3 Channels

Cat #: STJ-080
Alternative Name JZTX-X, Jingzhaotoxin F7-15.33
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Chilobrachys guangxiensis (Chinese earth tiger tarantula) (Chilobrachys jingzhao)
    Source Synthetic peptide
    MW: 3706 Da
    Purity: >98% (HPLC)
    Form Lyophilized Powder
    Effective concentration 50 – 500 nM
    Sequence LCSREGEFCYKLRKCCAGFYCKAFVLHCYRN - OH
    Modifications Disulfide bonds location- Cys2-15, Cys9-21 and Cys16-28
    Structure
    Molecular formula C164H242N46O41S6
    Activity Jingzhaotoxin-X inhibits the activation of Kv4.2 and Kv4.3 channels and exhibits long-lasting mechanical hyperalgesia in mice1.
    References-Activity
    1. Deng, M. et al. (2020) J. Venom Anim. Toxins Incl. Trop. Dis. 26, e20190043.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Protect from light and moisture.
    Solubility Soluble in water. Centrifuge all products before use (10000 g, 5 min). It is recommended to prepare fresh solutions in working buffers before use, or aliquot stock solutions reconstituted in distilled water and keep at -20°C. Upon use, dilute the stock solution in the desired working buffer. Prevent repeated thawing and freezing cycles.
    Storage of solutions Store up to one week at 4°C or up to 6 months at -20°C.
    Our bioassay
    • Alomone Labs Jingzhaotoxin-X inhibits KV4.2 channel currents heterologously expressed in Xenopus oocytes.
      Alomone Labs Jingzhaotoxin-X inhibits KV4.2 channel currents heterologously expressed in Xenopus oocytes.
      A. Representative time course of Jingzhaotoxin-X (#STJ-080) inhibition of KV4.2 channels current. Maximum current amplitudes were plotted as a function of time. Membrane potential was held at –100 mV, current was elicited by a 100 ms voltage step to 0 mV every 10 sec, and inhibited by application of 50 nM (green) and 500 nM (magenta) Jingzhaotoxin-X. 
      B. Superimposed traces of KV4.2 channel currents in the absence (control) and presence of 50 nM (green) and 500 nM (magenta) Jingzhaotoxin-X (taken from the recording in A).
    References - Scientific background
    1. Liao, Z. et al. (2007) Proteomics 11, 1892.
    2. Deng, M. et al. (2020) J. Venom Anim. Toxins Incl. Trop. Dis. 26, e20190043.
    3. Birnbaum, S.G. et al. (2004) Physiol. Rev. 84, 803.
    Scientific background

    Jingzhaotoxin-X (JZTX-X) is a 31 amino acid peptidyl toxin originally isolated from the venom of the Chinese earth tiger tarantula, Chilobrachys guangxiensis 1,2. JZTX-X is a selective blocker of voltage-gated potassium (Kv)4.2 and Kv4.3 channels2.

    Kv channels play key roles in human physiology and pathophysiology. Among the various Kv channels, the Shal-type (Kv4) family is characterized by rapid inactivation and activation, expressing the transient potassium currents within the heart, nervous system, and a subpopulation of smooth muscle cells3. Kv4.2 channel has been identified as a potent factor contributing to the A-type currents within the dorsal horn neurons, and reside at a crucial position for regulating pain processing. Kv4.3 channels are detected within the soma of a neuronal subset of the non-peptidergic nociceptive dorsal root ganglion (DRG), which plays a critical role in the regulation of neuron excitability. Down-regulation of the Kv4.3 channel in pain-sensing neurons can boost pain sensation. JZTX-X toxin was shown to induce long-lasting mechanical hyperalgesia in an animal model2.

    Target KV4.2 and KV4.3 Channels
    Peptide Content: 100%
    Last update: 29/08/2021

    Jingzhaotoxin-X (#STJ-080) is a highly pure, synthetic, and biologically active peptide toxin.

    For research purposes only, not for human use
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