Certificate of Analysis

KCa1.1 Channel Modulator Explorer Kit

A Screening Package of KCa1.1 Channel Modulators Economically Priced
Cat #: EK-132 13 Vials

Charybdotoxin Cat #: STC-325

Size: 0.1 mg MW: 4296 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
Charybdotoxin is a potent selective inhibitor of high conductance (maxi-K), different medium and small conductance Ca2+-activated K+ channels, as well as a voltage-dependent K+ channel (KV1.3)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Kaliotoxin-1 Cat #: STK-370

Size: 50 µg MW: 4150 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
Kaliotoxin-1 is a potent inhibitor of large conductance Ca2+-activated K+ channels (BKCa)1,2. Kaliotoxin-1 also binds the dendrotoxin sensitive voltage-dependent K+ channels (KV1.1, KV1.2 and KV1.3 nM)3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.

Iberiotoxin Cat #: STI-400

Size: 0.1 mg MW: 4230.9 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
50-100 nM.
Activity
Iberiotoxin is a potent selective blocker of the high conductance Ca2+-activated Kchannels (maxi-K).
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Isopimaric Acid Cat #: I-370

Size: 1 mg Source: Natural MW: 302 Purity: >99%
Origin
Natural product of unspecified plant origin.
Effective concentration
1-100 μM.
Activity
Isopimaric acid is a potent opener of large conductance Ca2+-activated K+ (BK) channels. 1 μM of Isopimaric acid doubles the current carried via BK channels. Isopimaric acid may increase the open probability by shifting the Ca2+ and voltage dependence to more hyperpolarized voltages1. The sensitivity is decreased in the Xenopus system compared to mammalian cells. BK channels activated by Isopimaric acid retain their charybdotoxin sensitivity.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO, ethanol, acetone or methanol. DMSO is suggested for biological experiments. DMSO solution should not exceed 0.03%. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

NS-11021 Cat #: N-335

Size: 5 mg Source: Synthetic MW: 511.24 Purity: >99%
Effective concentration
0.5-50 µM.
Activity
NS-11021 is a potent KCa1.1 channel agonist, enhancing KCa1.1 currents with EC50 of 0.4 µM, whilst having no effect on a variety of K+, Na+ and Ca2+ channels at 10 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Up to 100 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

NS-1619 Cat #: N-105

Size: 5 mg Source: Synthetic MW: 362.2 Purity: >95%
Effective concentration
100 nM - 100 μM.
Activity
NS-1619 is a potent and selective KCa1.1 (BK, slo, Large conductance Ca2+-activated K+) channel modulator (enhancer)1. NS-1619 was effective in eliciting and enhancing KCa1.1 channels in smooth muscle as well as in neurons with maximal effects at concentrations around 30 μM1,2. NS-1619 was used in vivo by intracarotid infusion and significantly enhanced blood tumor barrier permeability in the brain tumor area but not in normal brain tissue3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

NS19504 Cat #: N-235

Size: 5 mg Source: Synthetic MW: 269.2 Purity: >99%
Effective concentration
5 µM – 100 µM.
Activity
NS19504 is a KCa1.1 channel opener1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
50 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Paxilline Cat #: P-450

Size: 0.45 mg Source: Natural MW: 435.6 Purity: >98% (HPLC)
Origin
Penicillium paxilli.
Effective concentration
10-100 nM.
Activity
Paxilline is a selective blocker of high-conductance Ca2+-activated (Maxi-K) K+ channels.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO or acetone. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.

Penitrem A Cat #: P-650

Size: 0.65 mg Source: Natural MW: 634.2 Purity: >98%
Origin
Penicillium palitans.
Effective concentration
10-100 nM.
Activity
Penitrem A is a selective blocker of KCa1.1 (high-conductance Ca2+-activated K+, BK) channels1. Penitrem A increases the spontaneous release of endogenous GABA (3-aminobutyric acid), and aspartate2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO, methanol, acetone. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.

Pimaric Acid Cat #: P-270

Size: 1 mg Source: Natural MW: 302 Purity: >85%
Origin
Natural product of unspecified plant origin.
Effective concentration
1-10 µM.
Activity
Pimaric acid is a potent opener of large conductance Ca2+-activated K+ (BK) channels. 1 µM of Pimaric acid doubles the current carried via BK channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Organic solvents such as DMSO, ethanol, acetone or methanol. DMSO is suggested for biological experiments. DMSO solution should not exceed 0.03%. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to three months at -20°C.

Slotoxin Cat #: STS-410

Size: 0.1 mg MW: 4086 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide.
Effective concentration
10-100 nM.
Activity
Slotoxin inhibits the Maxi K+ (BKCa or mSlo, KCNMA1, KCa1.1) channels1. Slotoxin differentially blocks channels formed by the α1 subunit alone and channels formed by the α1 combined with auxiliary β subunits.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.

Tanshinone II-A sodium sulfonate Cat #: T-165

Size: 5 mg Source: Synthetic MW: 396.4 Purity: ≥98%
Effective concentration
10-200 µM.
Activity
Tanshinone II-A sodium sulfonate  is a KCa1.1 channel opener1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
10 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Verruculogen Cat #: V-500

Size: 0.51 mg Source: Natural MW: 511.23 Purity: >98%
Origin
Penicillium simplicissimum.
Effective concentration
10-100 nM.
Activity
Verruculogen is a selective blocker of KCa1.1 (BK) channel. It belongs to a set of tremorgenic indole alkaloids1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO, methanol or acetone.
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

For more information, please refer to individual certificate of analysis for each product.