KCNE1/IsK Blocking Peptide (#BLP-PC163) is the original antigen used for immunization during Anti-KCNE1 (IsK) Antibody (#APC-163) generation. The blocking peptide binds and ‘blocks’ Anti-KCNE1/IsK primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes:
- (C)RSKKLEHSHDPFN, corresponding to amino acid residues 68-80 of rat KCNE1 (Accession P15383).