KCNH2/HERG Blocking Peptide (#BLP-PC062) is the original antigen used for immunization during Anti-KCNH2 (HERG) Antibody (#APC-062) generation. The blocking peptide binds and ‘blocks’ Anti-KCNH2/HERG primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of KV11.1 (HERG)-expressing HEK-293 cells:
- Mouse frozen heart sections (1:300) (Teng, G.Q. et al. (2008) Circ. Res. 103, 1483.).
- GST fusion protein with the sequence DSLSQVSQFMACEELPPGAPELPQEGPTRRLSLPGQLGALTSQPLHRHGSDPGS, corresponding to amino acid residues 1106-1159 of human KV11.1 (HERG) (Accession Q12809).