KCNK12/THIK-2 (extracellular) Blocking Peptide (#BLP-PC169) is the original antigen used for immunization during Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169) generation. The blocking peptide binds and ‘blocks’ Anti-KCNK12/THIK-2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain synaptosomal fraction (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
- Western blot analysis of mouse lung lysate (lanes 1 and 3) and rat kidney membranes (lanes 2 and 4):
- Western blot analysis of human SH-SY5Y neuroblastoma cell lysate:
- Expression of KCNK12 (THIK-2) in rat hippocampusImmunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169), (1:400), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. THIK-2 immunoreactivity (green) in the rat hippocampal CA3 region is detected in neuronal soma (horizontal arrows) and in the dendrite initial segment (vertical arrows). Cell nuclei are stained with DAPI (blue).
- (C)ESPGEAEARARWG, corresponding to amino acid residues 61-73 of rat KCNK12 (Accession Q9ERS1).