Overview
Type: Synthetic peptide
Form: Lyophilized powder
KCNN1/KCa2.1 Blocking Peptide (#BLP-PC039) is the original antigen used for immunization during Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039) generation. The blocking peptide binds and ‘blocks’ Anti-KCNN1/KCa2.1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain cortex:1. Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200).
2. Anti-KCNN1 (KCa2.1, SK1) Antibody, preincubated with KCNN1/KCa2.1 Blocking Peptide (#BLP-PC039).- Mouse kidney sections (1:200) (Li, Y. et al. (2016) PLoS ONE 11, e0155006.).
Expression of KCa2.1 in rat parietal cortex.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. KCa2.1 immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the antibody with KCNN1 (KCa2.1, SK1) Blocking Peptide (BLP-PC039), suppressed staining. Cell nuclei are stained with DAPI (blue).
Expression of KCa2.1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region shows KCa2.1 immunoreactivity (green) in the pyramidal cell layer (P) and apical dendrites (arrows). B. Pre-incubation of the antibody with KCNN1 (KCa2.1, SK1) Blocking Peptide (BLP-PC039), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1 (Accession P70606).
Accession (Uniprot) Number P70606
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
