KT5720

A Selective PKA Inhibitor
    Cat #: K-190
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Nocardiopsis sp. (soil fungi).
    Source Semi-synthetic
    MW: 537
    Purity: >99% (HPLC)
    Effective concentration 100-500 nM.
    Structure
    • KT5720
    Chemical name (9R,10S,12S)-2,3,9,10,11,12-Hexahydro-10-hydroxy-9- meth yl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg:3',2',1'-kl]py rrolo[3,4- i][1,6]benzodiazocine-10-carboxylic acid, hexyl ester.
    Molecular formula C32H31N3O5.
    CAS No.: 108068-98-0.
    Activity KT5720 is a potent and specific inhibitor of PKA (IC50 = 56 nM)1.
    References-Activity
    1. Kase, H. et al. (1987) Biochem. Biopphys. Res. Commun142, 436.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
    Protect from light.
    Our bioassay
    • KT5720
      Alomone Labs KT5720 inhibits PKAS phosphorylation via PKA.
      NIH/C6 cells were preincubated for 45 min with different concentrations of KT5720 (#K-190) and then stimulated with 10 µM Forskolin (#F-500) for 20 min. Cell proteins were resolved by SDS-PAGE and probed with antiphospho-(Ser/Thr) PKAS (PKA substrate).
    • KT5720
      Alomone Labs KT5720 inhibits NGF-mediated survival of serum-deprived PC12 cells.
      Dose-response curve of KT5720 (#K-190) inhibition of Native mouse NGF 2.5S protein (>95%) (#N-100), (50 ng/ml) mediated survival of serum-deprived PC12 cells. Cell viability was determined by XTT method and normalized to control. IC50 was determined at 287.4 nM.
    References - Scientific background
    1. Kase, H. et al. (1987) B.B.R.C. 142, 436.
    2. Mohri, T. et al. (1998) Cell. Struct. Funct. 23, 255.
    3. Audesirk, G. et al. (1997) Brain Res. Dev. Brain Res. 102, 247.
    4. Okada, Y. et al. (1995) J. Neurosci. 15, 3053.
    5. Vianna, M.R. et al. (1999) Behav. Pharmacol. 10, 223.
    6. Huang, Y.Y. et al. (2000) J. Neurosci. 20, 6317.
    7. Kim, M.S. et al. (2002) Biochem. Biophys. Res. Commun. 291, 663.
    Scientific background KT5720 is a semi-synthetic derivative of K525a. It is a cell-permeable, selective inhibitor of cAMP-dependent protein kinase (PKA; IC50 = 56 nM). No significant effect on protein kinase C (PKC), protein kinase G (PKG) or myosin light chain kinase (MLCK), was observed.1 KT5720 was used to examine the role of PKA in various cellular events such as cell adhesion,2 neurite branching,3 anterograde axonal transport,4 memory consolidation,5,6 and regulation of transcription by cyclic AMP-dependent response element-binding protein (CREB).7
    Last update: 24/01/2020

    KT5720 (#K-190) is a highly pure, semi-synthetic, and biologically active compound.

    For research purposes only, not for human use

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    Scientific Background

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