Kvβ2 Blocking Peptide (#BLP-PC117) is the original antigen used for immunization during Anti-KVβ2 Antibody (#APC-117) generation. The blocking peptide binds and ‘blocks’ Anti-Kvβ2 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain lysate (lanes 1 and 3) and membranes (lanes 2 and 4):1,2. Anti-KVβ2 Antibody (#APC-117), (1:200).
3,4. Anti-KVβ2 Antibody, preincubated with Kvβ2 Blocking Peptide (#BLP-PC117).
- Western blot analysis of human Jurkat T cells:1. Anti-KVβ2 Antibody (#APC-117), (1:200).
2. Anti-KVβ2 Antibody, preincubated with Kvβ2 Blocking Peptide (#BLP-PC117).
- Expression of KVβ2 in mouse cerebellumImmunohistochemical staining of mouse cerebellum using Anti-KVβ2 Antibody (#APC-117). A. KVβ2 appears adjacent to Purkinje cells (green). B. Staining of GABAergic cells with mouse anti-parvalbumin (PV, red). C. Confocal merge of KVβ2 and PV demonstrates the presence of KVβ2 adjacent to Purkinje cells.
- SPGMIYSTRYGSPKR(C), corresponding to amino acid residues 20-34 of rat KCNAB2 (Accession P62483).