Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv1.1/KCNA1 (extracellular) Blocking Peptide (#BLP-PC161) is the original antigen used for immunization during Anti-KV1.1 (KCNA1) (extracellular) Antibody (#APC-161) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.1/KCNA1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):1,2. Anti-KV1.1 (KCNA1) (extracellular) Antibody (#APC-161), (1:200).
3,4. Anti-KV1.1 (KCNA1) (extracellular) Antibody, preincubated with Kv1.1/KCNA1 (extracellular) Blocking Peptide (#BLP-PC161).
Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate:1. Anti-KV1.1 (KCNA1) (extracellular) Antibody (#APC-161), (1:200).
2. Anti-KV1.1 (KCNA1) (extracellular) Antibody, preincubated with Kv1.1/KCNA1 (extracellular) Blocking Peptide (#BLP-PC161).
Properties
Sequence
- (C)KDDKDFTGTIHRID, corresponding to amino acid residues 193-206 of rat KV1.1 (Accession P10499).
Accession (Uniprot) Number P10499
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
