Kv1.1/KCNA1 Blocking Peptide (#BLP-PC009) is the original antigen used for immunization during Anti-KV1.1 (KCNA1) Antibody (#APC-009) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.1/KCNA1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Rat brain sections.
Mouse sciatic nerve (1:200) (Zhou, L. et al. (1998) J. Neurosci. 18, 7200.).
- GST fusion protein with the sequence HRETEGEEQAQLLHV SSPNLASDSDLSRRSSSTISKSEYMEIEEDMNNSIAHYRQANIRTGNCTTADQNCVNKSKLLTDV, corresponding to amino acid residues 416-495 of mouse KV1.1 (Accession P16388).