KV1.2 (KCNA2) Channel Antibody and Membrane Fractions Kit

Kit Contains Anti-KV1.2 (KCNA2) Antibody and all Controls Necessary for Robust Western Blot Analysis
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
Cat #: LK-101
Sizes: 4 Vials
Last update: 25/11/2018

KV1.2 (KCNA2) Channel Overexpressed Membrane Fractions (#LX-101) are Xenopus oocyte membrane fractions overexpressing the KV1.2 channel. KV1.2 (KCNA2) Channel Overexpressed Membrane Fractions are your positive control for validating Alomone Labs Anti-KV1.2 (KCNA2) Antibody (#APC-010).

Overexpressed Membrane Fractions are:
✓ Lyophilized powder
✓ Economical
✓ Shipped at room temperature (no need for dry ice and extra shipping costs)
✓ User-friendly & time-saving. Just add water, sample buffer and load your gel

For research purposes only, not for human use
Compounds
Product Name Cat # Size
Anti-KV1.2 (KCNA2) Antibody
APC-010 1 x 0.2 ml
KV1.2 (KCNA2) Channel Overexpressed Membrane Fractions
LX-101 2 x 0.1 ml
Note

Kit includes:
1 x 40 µg Anti-KV1.2 (KCNA2) Antibody control antigen
1 x 0.1 ml lyophilized non-injected Xenopus oocyte membrane lysate

Scientific Background
Scientific Background

KV1.1 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.1 was the first mammalian KV channel to be cloned from mouse brain.1 Eight Shaker-related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2

A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.

The structure of KV1.1 channel is similar to all KV channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain.2

The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in retina and pancreas.2 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. Mutations in the coding of KV1.1 gene were discovered in Episodic Ataxia patients.3

KV1.1 channels are sensitive to low doses of TEA (0.3 mM) and 4-AP (0.29 mM), the “classical” non-selective potassium channel blockers.

Several venomous toxins from snakes, scorpions and sea anemones are potent blockers (affecting the channels in the nanomolar range) of KV1.1 channels. Among these the most potent and selective are α-Dendrotoxin (#D-350, 0.4-4 nM) and δ-Dendrotoxin (#D-380, 0.03-1.8 nM), Dendrotoxin-K (#D-400, 0.03 nM), Agitoxin-2 (#STA-420, 0.044 nM) and Hongotoxin-1 (#RTH-400, 0.031 nM).4

References
  1. McKinnon, D. (1989) J. Biol. Chem. 264, 8230.
  2. Gutman, G.A. et al. (2005) Pharmacol. Rev57, 473.
  3. Long S.B. et al. (2005) Science 309, 897.
  4. Bogin, O. (2006) Modulator 21, 28.
Related Products