Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv1.2/KCNA2 (extracellular) Blocking Peptide (#BLP-PC162) is the original antigen used for immunization during Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.2/KCNA2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat heart membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):1-3. Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162), (1:200).
4-6. Anti-KV1.2 (KCNA2) (extracellular) Antibody, preincubated with Kv1.2/KCNA2 (extracellular) Blocking Peptide (#BLP-PC162).
Properties
Sequence
- (C)RDENEDMHGGGVT, corresponding to amino acid residues 189-201 of rat KV1.2 (Accession P63142).
Accession (Uniprot) Number P63142
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
