Free shipping starts now, no minimum, no coupons required!

Accessibility

Accessibility Statement

 
Introduction
The internet today constitutes the largest database of free information for all users, including users with disabilities. As such, we place great emphasis on providing equal opportunity for people with disabilities to be able to use the information on our website and enjoy a better browsing experience. 
We aim to ensure that digital services are accessible to people with disabilities, and therefore we have invested large resources in order to simplify the use of the website for people with disabilities as much as possible, out of the belief that every person deserves the right to live with equality, dignity, convenience, and independence. 
 
Using the website accessibility program
This website is run by the accessibility program of the "Accessible with a Click" company and is run via a designated accessibility server. The program allows the website to follow the guidelines for internet content accessibility WCAG 2.0 to level AA. The program is subject to the conditions of use of the manufacturer. Warrantee of use applies to the website owners and/or their representative, including the content displayed in the website, as subject to the conditions of use. 
 
How does accessibility work on the website?

The website has an accessibility menu. Clicking on the menu opens accessibility buttons. After choosing a subject in the menu, waiting time is necessary for the page to upload. 

What is in the menu?

 

✓ Ability to navigate with the keyboard
✓ Increasing and decreasing the website fonts
✓ Changing color contrast based on dark backgrounds
✓ Changing color contrast based on light backgrounds
✓ Adapting the website to color blind people
✓ Underlining links throughout the website

 

Clarification

Despite our efforts to enable website browsing for all the website pages, there may be website pages that haven't been made accessible yet or may lack a suitable technical solution.
We are continuing our efforts to enhance the accessibility of the website as much as possible, out of our moral obligation to enable the use of the website for the population as a whole, including people with disabilities. 

Navigation

Alomone Labs Alomone Labs

Order Summary

Home

main

    • column
      • Ion Channels
    • column
      • G-Protein Coupled Receptors
      • Transporters, Exchangers and Pumps
    • column
      • Neurotrophic Factors and Receptors
      • Neural Signaling
    • column
      • Cell Imaging Tools
      • Research Areas
  • Home
  • Products
    • column
      • New Products
      • All Products
      • Research Areas
      • Select Targets
    • column
      • Ion Channels
      • G-Protein Coupled Receptors
      • Transporters, Exchangers and Pumps
      • Controls
    • column
      • Neurotrophic Factors and Receptors
      • Neural Signaling
      • Cell Imaging Tools
      • Kits & Packs
    • column
      • Guinea Pig Polyclonal Antibodies
      • Multiplex Staining Products
      • Live Cell Imaging Products
      • Live Cell Flow Cytometry Products
  • Free Sample Program
  • Services
    • column
      • Custom Services
      • Online Quotes New
      • Ordering & Shipping
  • Resources
    • column
      • NaV Channels
      • Microglia Markers
      • CaV Channels
      • ASICs
      • nAChR
      • TRPV1
      • NMDA Receptor
      • KCa Channels
    • column
      • Tips
      • Protocols
      • Blog
      • Articles
    • column
      • FAQs and Troubleshooting
      • Citations Search Tool
      • Download Protocol Book
  • About
    • column
      • About Alomone Labs
      • Why Alomone Labs?
  • Contact
    • column
      • Contact Us
      • Subscribe – Newsletters and Email Updates
Return to Content
Home › Products › Ion Channels › K+ Channels › Voltage-Gated K+ Channels › Antibodies to KV Channels › Anti-KV1.3 (KCNA3) (extracellular) Antibody › Blocking Peptide

Certificate of Analysis

Kv1.3/KCNA3 (extracellular) Blocking Peptide

Back to product page SDS
Cat #: BLP-PC101
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: wb, ihc

Application key:

WB- Western blot, IHC- Immunohistochemistry
For research purposes only, not for human use

Properties

Sequence
  • KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001).
Accession (Uniprot) Number P22001
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 3 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Demonstration of Pre-adsorption control
  • Western blot analysis of rat brain membranes:  
    Western blot analysis of rat brain membranes:  
    1. Anti-KV1.3 (KCNA3) (extracellular) Antibody  (#APC-101), (1:500).  
    2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
  • Western blot analysis of human Jurkat T cells:
    Western blot analysis of human Jurkat T cells:
    1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
    2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
  • Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:200).
    3,4. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
  • Mouse frozen brain sections (1:100). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).
Last update: 08/01/2023

Specific Use it with:

  • Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.

    Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101)
  • Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV1.3 potassium channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

    Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP (formerly AGP-005))

General Protocols

  • Blocking Peptides – Controls for better results
  • Blocking Peptide Protocol for Western Blot (WB)
Peptide Blocking Overview Diagram

Shipping and Ordering Information

Free International Shipping

Products are lyophilized and ship at room temperature - FREE OF CHARGE.

We ship worldwide within 24 hours of our next business day.

For more country-specific shipping and contact information see Ordering & Shipping.


Free Sample Program

Not sure whether the product fits your experimental setup?

Try it first!


Alomone Guarantee

We offer 100% guarantee on all our products. If our products do not perform as stated, please contact our technical support staff.

Alomone Guarantee information

Need Help With This Product?

Our reagent specialists are here to help you find the best product for your application. Please message us and we will be happy to help you find the right product for the job.

Contact Us

Contact Us

© 2023 Alomone Labs. All Rights Reserved.

Terms of Service Privacy Policy
Scroll to top