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Certificate of Analysis

Kv1.3/KCNA3 (extracellular) Blocking Peptide

Overview

Cat #: BLP-PC101
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Applications

Applications

Blocking
  • Western blot analysis of rat brain membranes:  
    Western blot analysis of rat brain membranes:  
    1. Anti-KV1.3 (KCNA3) (extracellular) Antibody  (#APC-101), (1:500).  
    2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
  • Western blot analysis of human Jurkat T cells:
    Western blot analysis of human Jurkat T cells:
    1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
    2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
  • Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).
  • Western blot analysis of human Jurkat T cell leukemia cell lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of human Jurkat T cell leukemia cell lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1-2. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B), (1:1000).
    3-4. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
  • Mouse frozen brain sections (1:400).
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#AGP-005), (1:200).
    3,4. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
  • Mouse frozen brain sections (1:100). Following a broad screen of secondary antibodies, the following was used for this application: #106-485-006 (Jackson ImmunoResearch).

Properties

Properties

Sequence
  • KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Standard Quality Control Of Each Lot Western blot analysis.
Last update: 23/08/2020
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