Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167) is the original antigen used for immunization during Anti-KV1.4 (extracellular) Antibody (#APC-167) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.4 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain lysate:1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400).
2. Anti-KV1.4 (extracellular) Antibody, preincubated with Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167).
Western blot analysis of mouse brain lysate:1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:200).
2. Anti-KV1.4 (extracellular) Antibody, preincubated with Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167).
Expression of KV1.4 in rat parietal cortexImmunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. KV1.4 staining (red) appears in several neuronal-outlined cells (arrows). Nuclei were stained with DAPI (blue).
Properties
Sequence
- (C)GHSRLLNDTSAPHLE, corresponding to amino acid residues 348 - 362 of rat KV1.4 (Accession P15385).
Accession (Uniprot) Number P15385
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
