Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv1.5/KCNA5 (extracellular) Blocking Peptide (#BLP-PC150) is the original antigen used for immunization during Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.5/KCNA5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150), (1:200).
2. Anti-KV1.5 (KCNA5) (extracellular) Antibody, preincubated with Kv1.5/KCNA5 (extracellular) Blocking Peptide (#BLP-PC150).
Expression of KV1.5 channels in rat cerebellumImmunohistochemical staining of rat cerebellum with Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150), (1:200). A. KV1.5 (green) appears in both the soma of Purkinje cells (horizontal arrows) and in Purkinje dendrites (vertical arrows). B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti-parvalbumin antibody (red). C. Merge of the two images demonstrates partial colocalization (white arrows).
Properties
Sequence
- (C)DERELLRHPPVP(K), corresponding to amino acid residues 268-279 of rat KV1.5 (Accession P19024).
Accession (Uniprot) Number P19024
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
