Kv1.5/KCNA5 (extracellular) Blocking Peptide (#BLP-PC150) is the original antigen used for immunization during Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.5/KCNA5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat brain membranes:
- Expression of KV1.5 channels in rat cerebellumImmunohistochemical staining of rat cerebellum with Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150), (1:200). A. KV1.5 (green) appears in both the soma of Purkinje cells (horizontal arrows) and in Purkinje dendrites (vertical arrows). B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti-parvalbumin antibody (red). C. Merge of the two images demonstrates partial colocalization (white arrows).
- (C)DERELLRHPPVP(K), corresponding to amino acid residues 268-279 of rat KV1.5 (Accession P19024).