Overview
Cat #: BLP-PC150
Type: Synthetic peptide
Form: Lyophilized powder
Kv1.5/KCNA5 (extracellular) Blocking Peptide (#BLP-PC150) is the original antigen used for immunization during Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150) generation. The blocking peptide binds and ‘blocks’ Anti-Kv1.5/KCNA5 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
Application key:
WB-western blot, IHC-Immunohistochemistry
For research purposes only, not for human use
Applications
Demonstration of Pre-adsorption control
- Western blot analysis of rat brain membranes:1. Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150), (1:200).
2. Anti-KV1.5 (KCNA5) (extracellular) Antibody, preincubated with Kv1.5/KCNA5 (extracellular) Blocking Peptide (#BLP-PC150). - Expression of KV1.5 channels in rat cerebellumImmunohistochemical staining of rat cerebellum with Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150), (1:200). A. KV1.5 (green) appears in both the soma of Purkinje cells (horizontal arrows) and in Purkinje dendrites (vertical arrows). B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti-parvalbumin antibody (red). C. Merge of the two images demonstrates partial colocalization (white arrows).
Properties
Sequence
Accession (Uniprot) Number P19024
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
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