Overview
Type: Synthetic peptide
Form: Lyophilized powder
Kv3.1b/KCNC1 Blocking Peptide (#BLP-PC014) is the original antigen used for immunization during Anti-KV3.1b (KCNC1) Antibody (#APC-014) generation. The blocking peptide binds and ‘blocks’ Anti-Kv3.1b/KCNC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membranes:1. Anti-KV3.1b (KCNC1) Antibody (#APC-014), (1:200).
2. Anti-KV3.1b (KCNC1) Antibody, preincubated with Kv3.1b/KCNC1 Blocking Peptide (#BLP-PC014).- Rat brain sections. Mouse medial nucleus of the trapezoid body (MNTB) (1:500) (Leao, K.E. et al. (2010) Eur. J. Neurosci. 32, 1658.).
Properties
Sequence
- CKESPVIAKYMPTEAVRVT, corresponding to amino acid residues 567-585 of rat KV3.1b (Accession P25122).
Accession (Uniprot) Number P25122
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
