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Certificate of Analysis

Kv4.2 Blocking Peptide

Overview

Cat #: BLP-PC023
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Properties

Properties

Sequence
  • (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2 (Accession Q63881).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Applications

Blocking
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-KV4.2 Antibody (#APC-023), (1:200).
    2. Anti-KV4.2 Antibody, preincubated with Kv4.2 Blocking Peptide (#BLP-PC023).
  • Rat brain sections.

    Human myometrium sections (1:50) (Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.).
  • Mouse and rat brain frozen sections, (1:60).
  • Expression of KV4.2 in substantia nigra
    Expression of KV4.2 in substantia nigra
    Immunohistochemical staining of perfusion-fixed frozen mouse and rat brain sections using Anti-KV4.2-ATTO Fluor-633 Antibody (#APC-023-FR), (1:60). A. Mouse brain. B. Rat brain. KV4.2 staining (magenta) in both sections is detected in cells with neuronal outline (arrows). DAPI is used as the counterstain (blue).
  • Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human U-87 MG glioblastoma cell lysate (lanes 3 and 6):
    Western blot analysis of mouse brain lysate (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and human U-87 MG glioblastoma cell lysate (lanes 3 and 6):
    1-3. Guinea pig Anti-KV4.2 Antibody (#AGP-038), (1:500).
    4-6. Guinea pig Anti-KV4.2 Antibody, preincubated with Kv4.2 Blocking Peptide (#BLP-PC023).
  • Mouse and rat brain sections (1:400).
Last update: 12/08/2021
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