Certificate of Analysis

KV4 Channel Blocker Explorer Kit

A Screening Package of KV4 Channel Blockers Economically Priced
Cat #: EK-130 14 Vials

AmmTx3 Toxin Cat #: STA-305

Size: 0.1 mg MW: 3822 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-5 µM.
Activity
AamTx3 Toxin is a blocker of A-type voltage-gated K+ channels as well as ERG1, KV11.1 and KCNH2 K+ channels1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Dapoxetine hydrochloride Cat #: D-175

Size: 10 mg Source: Synthetic MW: 341.8 Purity: >99.5%
Effective concentration
5-300 µM.
Activity
Dapoxetine blocks with an IC50 of 5.3 μM for cloned KV4.3 and 11.6 μM for KV1.5 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
200 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

(S)-Duloxetine hydrochloride Cat #: D-170

Size: 25 mg Source: Synthetic MW: 333.9 Purity: >99%
Effective concentration
1-300 µM.
Activity
(S)-Duloxetine hydrochloride blocks KV4.3 channels with an IC50 of 8.4 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
200 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Fluoxetine hydrochloride Cat #: F-155

Size: 50 mg Source: Synthetic MW: 345.8 Purity: >99.5%
Effective concentration
5-300 µM.
Activity
Fluoxetine is a blocker of KV4.3 channels with an IC50 of 11.8 μM on cloned channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
200 mM in DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Guangxitoxin-1E Cat #: STG-200

Size: 0.1 mg MW: 3948.7 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-100 nM.
Activity
Guangxitoxin-1E is a gating modifier of KV2.1 (KCNB1, IC50 of 1 nM), KV2.2 (KCNB2, IC50 of 3 nM) and KV4.3 (KCND3, IC50 of 10-20 fold higher concentration) channels. In pancreatic β cells it enhances glucose-stimulated insulin secretion by broadening the cell action potential and enhancing calcium oscillations1,2. The physiological modulation of KV2.1 channels during glucose-induced insulin secretion is MgATP-mediated3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Heteropodatoxin-2 Cat #: STH-340

Size: 0.1 mg MW: 3413 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
100-500 nM.
Activity
Heteropodatoxin-2 is an inhibitor of voltage-gated Kchannels of the KV4 family. Inhibition of KV4.3 and KV4.2 is strongly voltage-dependent, while inhibition of KV4.1 shows less voltage-dependence. Lacks affinity for KV1.4, KV2.1 and KV3.41,2. Also blocks Ca2+ channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.

Jingzhaotoxin-V Cat #: STJ-050

Size: 50 µg MW: 3605.4 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
5-10 nM.
Activity
Jingzhaotoxin-V inhibits TTX-resistant and TTX-sensitive Na+ channels in DRG neurons. It also highly inhibits KV4.2 and KV4.3 K+ channels and weakly inhibits KV2.1 K+ channels. It has no effect on KV1.2, KV1.3 or KV1.4 K+ channels1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Jingzhaotoxin-XII Cat #: STJ-100

Size: 50 µg MW: 3665 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
200 nM - 2 µM.
Activity
Jingzhaotoxin-XII inhibits KV4.1 and NaV1.5 voltage-gated channels1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Pandinotoxin Kα Cat #: STP-500

Size: 50 µg MW: 4033 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-200 nM.
Activity
Pandinotoxin Kα is an A-type and Shaker-related K+ channel blocker1-4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Phrixotoxin-1 Cat #: STP-700

Size: 50 µg MW: 3549 Da. Purity: >99% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
10-100 nM.
Activity
Phrixotoxin-1 specifically and reversibly blocks KV4.2 and KV4.3 channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10,000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Phrixotoxin-2 Cat #: STP-710

Size: 50 µg MW: 3923 Da. Purity: >99% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
100-500 nM.
Activity
Phrixotoxin-2 specifically and reversibly blocks KV4.21.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10,000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Rosiglitazone Cat #: R-125

Size: 10 mg Source: Synthetic MW: 357.43 Purity: >97%
Effective concentration
2-200 µM.
Activity
Rosiglitazone blocks KV4.3 channels with an IC50 = 24.5 μM1, Kir6.1 channels with as IC50 = 2 μM2 and blocks TRPM3 channels with IC50 = 5-10 μM3. At high concentrations Rosiglitazone activates TRPC5 channels3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in DMSO . Centrifuge all product preparations before use (10000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

SNX-482 Cat #: RTS-500

Size: 25 µg MW: 4495 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
10-100 nM.
Activity
SNX-482 blocks R-type (CaV2.3) and P/Q-type (CaV2.1) voltage-gated Ca2+ channels1. It also blocks A-type K+ channels with an IC50 < 3 nM2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Trifluoperazine dihydrochloride Cat #: T-175

Size: 1 g Source: Synthetic MW: 480.4 Purity: >99%
Effective concentration
5-300 µM.
Activity
Trifluoperazine dihydrochloride blocks KV4.3 with an IC50 ~8 μM on cloned channels. It also inhibits D1 and D2 dopamine receptors1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
100 mM in H2O. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

For more information, please refer to individual certificate of analysis for each product.

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