Certificate of Analysis

L-Type CaV Channel Blocker Explorer Kit

A Screening Package of L-Type CaV Channel Blockers Economically Priced
Cat #: EK-103 24 Vials

Amlodipine Cat #: A-110

Size: 250 mg Source: Synthetic MW: 408.9 Purity: >96%
Effective concentration
1-100 µM.
Activity
Amlodipine besylate is a dihydropyridine that acts as an L-type voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2. Amlodipine besylate inhibits L- and T-type CaV channels with similar potency3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Amlodipine besylate Cat #: A-120

Size: 10 mg Source: Synthetic MW: 567.1 Purity: >99%
Effective concentration
1-100 μM.
Activity
Amlodipine besylate is a dihydropyridine that acts as an L-type voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2. Amlodipine besylate inhibits L- and T-type CaV channels with similar potency3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Azelnidipine Cat #: A-135

Size: 5 mg Source: Synthetic MW: 582.65 Purity: >98%
Effective concentration
1 – 100 µM.
Activity
0.3-1 μM inhibited guinea-pig vascular smooth muscle1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Benidipine hydrochloride Cat #: B-120

Size: 5 mg Source: Synthetic MW: 542 Purity: >99% (HPLC)
Effective concentration
1-100 µM.
Activity
Benidipine hydrochloride is a state-dependent Ca2+ channel blocker. It inhibits N-type and P/Q-type channels with IC50 of ~30 µM and is an order of magnitude more potent towards L-type channels, exhibiting the following IC50: 14 µM at -80mV and 5 µM at -60 mV holding potentials1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Calcicludine Cat #: SPC-650

Size: 70 µg MW: 6979 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
500 nM.
Activity
Calcicludine is a potent and specific antagonist of neuronal L-type CaV channels1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Calciseptine Cat #: SPC-500

Size: 70 µg MW: 7036 Da. Purity: >98% Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
500 nM.
Activity
Calciseptine is a specific antagonist of L-type CaV channels1.
Storage before reconstitution
Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Diltiazem hydrochloride Cat #: D-135

Size: 1 g Source: Synthetic MW: 451 Purity: >98%
Effective concentration
10 µM - 1 mM.
Activity
IC50 was 33 µM on CaV1.2 channels in expression systems1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Felodipine Cat #: F-105

Size: 5 mg Source: Synthetic MW: 384.25 Purity: >99%
Effective concentration
1-10 μM.
Activity
Felodipine is a dihydropyridine that acts as L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Flavoxate hydrochloride Cat #: F-140

Size: 10 mg Source: Synthetic MW: 427.9 Purity: >96%
Effective concentration
1-100 µM.
Activity
Flavoxate caused a concentration-dependent reduction of the K+-induced contraction of human urinary bladder. In human detrusor myocytes, flavoxate inhibited the peak amplitude of voltage-dependent nifedipine-sensitive inward Ba2+ currents in a voltage- and concentration-dependent manner (K10 µM), and shifted the steady-state inactivation curve of Ba2+ currents to the left at a holding potential of -90 mV1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Water. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Flunarizine dihydrochloride Cat #: F-110

Size: 1 g Source: Synthetic MW: 477.4 Purity: >99% (HPLC)
Effective concentration
1-200 μM.
Activity
Flunarizine dihydrochloride is a diphenylmethylpiperazine L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat migraine1. Flunarizine dihydrochloride inhibits L- and T-type CaV channels in guinea pig ventricular cells with similar potency and in a use-dependent manner2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

FS-2 Cat #: F-700

Size: 70 µg MW: 7026 Da. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Natural peptide isolated from Dendroaspis polylepis polylepis (Black mamba snake).
Effective concentration
100 nM - 2 ֲµM.
Activity
FS-2 is an L-type CaV channel blocker1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Isradipine Cat #: I-100

Size: 5 mg Source: Synthetic MW: 371.4 Purity: >99%
Effective concentration
50 nM - 5 μM.
Activity
Isradipine is a dihydropyridine that acts as an L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2. Isradipine inhibits L-type channels expressed in Xenopus oocytes with IC50 = 0.2 μM at a holding potential of -80 mV and the inhibition is state dependent and increases with reduced membrane hyperpolarization2. Isradipine, was also shown recently to putatively be an effective treatment for Parkinson’s disease, by affecting particularly the excitability of certain CaV1.3 expressing dopaminergic neurons3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

L-651,582 Cat #: L-110

Size: 5 mg Source: Synthetic MW: 424.7 Purity: >99%
Effective concentration
0.5-200 μM.
Activity
L-651,582 inhibits CaV channels as well as receptor activated Ca2+ signals in guinea pig atrial cells1, thus it is an L-type, voltage-gated Ca2+ channel blocker with additional antiproliferative activities2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Lercanidipine hydrochloride Cat #: L-115

Size: 5 mg Source: Synthetic MW: 648.2 Purity: >98%
Effective concentration
1-100 µM.
Activity
10 µM were effective in abolishing Ang II mediated smooth muscle contraction1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Lomerizine dihydrochloride Cat #: L-125

Size: 50 mg Source: Synthetic MW: 541.5 Purity: >99%
Effective concentration
0.5-100 µM.
Activity
Lomerizine dihydrochloride inhibited CaV currents in PC12 cells with IC50 of 1.9 µM1. It inhibits L-type Ca2+ channels2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Loperamide hydrochloride Cat #: L-100

Size: 5 g Source: Synthetic MW: 513.5 Purity: >99%
Effective concentration
1-100 µM.
Activity
Loperamide hydrochloride inhibits (IC50 = 2.5 μM) HVA CaV in hippocampal cells. It acts as a μ-opioid receptor positive modulator and also affects NMDA receptors1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

(±)-Naringenin Cat #: N-110

Size: 1 g Source: Synthetic MW: 272.25 Purity: >98%
Effective concentration
100 µM - 1mM.
Activity
Vascular L-type blocker with IC50 = 100 µM1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Nicardipine HCl Cat #: N-125

Size: 1 g Source: Synthetic MW: 515.9 Purity: >99%
Effective concentration
0.1-100 μM.
Activity
Nicardipine HCl is a dihydropyridine that acts as an L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2. Nicardipine inhibits L- and T-type CaV channels with similar potency3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Nifedipine Cat #: N-120

Size: 1 g Source: Synthetic MW: 346.3 Purity: >99.8%
Effective concentration
0.1 -10 μM.
Activity
Nifedipine, the prototype of dihydropyridines (DHPs), is an L-type voltage-gated Ca2+ channel selective blocker1. Nifedipine scarcely blocks the two N- and P/Q-type channels in Xenopus oocytes, whereas the effect of 10 μM Nifedipine on the L- type channel, at a holding potential of −80 mV, is approximately 65% block1. In the clinic Nifedipine is used for the prophylaxis of angina pectoris symptoms and as an anti-hypertensive agent2-3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Nimodipine Cat #: N-150

Size: 250 mg Source: Synthetic MW: 418.4 Purity: >99%
Effective concentration
0.1-100 μM.
Activity
Nimodipine is a dihydropyridine that acts as an L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2. Nimodipine inhibits L- type CaV channels with IC50 of ~10 μM and has little effect on other CaV channels at this concentration3,4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Nisoldipine Cat #: N-160

Size: 50 mg Source: Synthetic MW: 388.4 Purity: >98%
Effective concentration
10 nM - 1000 µM.
Activity
10 nM completely inhibited L-type currents expressed in HEK-293 cells1. The IC50 value of nisoldipine was 2.2 and 0.084 μM in oocytes injected with α1β and α1β α2, respectively2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Nitrendipine Cat #: N-155

Size: 50 mg Source: Synthetic MW: 360.4 Purity: >98%
Effective concentration
1-200 µM.
Activity
10 μM inhibited 50% of L-type current expressed in Xenopus oocytes, with no effect on T-type channels.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

SR 33805 oxalate Cat #: S-105

Size: 5 mg Source: Synthetic MW: 654.77 Purity: >96%
Effective concentration
1 nM - 10 μM.
Activity
SR 33805, a fantofarone derivative, is a potent Ca2+ channel antagonist1. It binds allosterically to the α1-subunit of L-type Ca2+ channels (Kd = 20 pM), at a site distinct from other types of blockers2. It potently inhibits the Ca2+ channel in primary mouse cardiac myocytes cultures with IC50 values ranging from 4 to 33 nM3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

Verapamil HCl Cat #: V-100

Size: 1 g Source: Synthetic MW: 491.1 Purity: >99%
Effective concentration
1-250 ?M.
Activity
Verapamil HCl is a phenylalkylamine that acts as an L-type, voltage-gated Ca2+ channel blocker and is used in the clinic to treat hypertension1,2. Verapamil block of L-type channels is dependent on the membrane potential. The IC50 values at a holding potential of -80 or -40 mV are 250 and 15.5 μM, respectively3. In Xenopus oocytes, Verapamil inhibits L-type currents in an activity dependent manner4.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Up to 50mM in water, DMSO and other solvents. Mild agitation and/or warming my help dissolve the product at higher stock concentrations. Centrifuge all product preparations before opening/handling (10000 x g 5 min).
Storage after reconstitution
Up to four week at 4°C or three months at -20°C.

For more information, please refer to individual certificate of analysis for each product.