Overview
Type: Synthetic peptide
Form: Lyophilized Powder
LAT2 (SLC7A8) (extracellular) Blocking Peptide (#BLP-NT108) is the original antigen used for immunization during Anti-LAT2 (SLC7A8) (extracellular) Antibody (#ANT-108) generation. The blocking peptide binds and ‘blocks’ Anti-LAT2 (SLC7A8) (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain synaptosomal fraction (lanes 2 and 4):1-2. Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200).
3-4. Anti-LAT2 (SLC7A8)(extracellular) Antibody, preincubated with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108).
Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 4), human MDA-MB-231 breast adenocarcinoma cell line lysates (lanes 2 and 5) and human MCF-7 breast adenocarcinoma cell line lysates (lanes 3 and 6):1-3. Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200).
4-6. Anti-LAT2 (SLC7A8)(extracellular) Antibody, preincubated with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108).
Expression of SLC7A8 in mouse hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus region, showed immunoreactivity of SLC7A8 (green) in the hilus (H). B. Pre-incubation of the antibody with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- Peptide CKGEFFWLEPKNAFEN, corresponding to amino acid residues 211 - 226 of rat SLC7A8 (Accession Q9WVR6).
Accession (Uniprot) Number Q9WVR6
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
