LAT3/SLC43A1 (extracellular) Blocking Peptide (#BLP-NT193) is the original antigen used for immunization during Anti-LAT3/SLC43A1 (extracellular) Antibody (#ANT-193) generation. The blocking peptide binds and ‘blocks’ Anti-LAT3/SLC43A1 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat lung (lanes 1 and 3) and mouse lung (lanes 2 and 4) lysates:
- Western blot analysis of human chronic myelogenous leukemia K562 (lanes 1 and 3) and human prostate carcinoma LN-CaP (lanes 2 and 4) cell lines lysates:1, 2. Anti-LAT3 (SLC43A1) (extracellular) Antibody (#ANT-193), (1:200).
3, 4. Anti-LAT3 (SLC43A1) (extracellular) Antibody, preincubated with LAT3/SLC43A1 (extracellular) Blocking Peptide (#BLP-NT193).
- (C)GKERETNEQRQKVE, corresponding to amino acid residues 336-349 of mouse LAT3 (SLC43A1) (Accession Q8BSM7).