Overview
Type: Synthetic peptide
Form: Lyophilized powder
LPAR1/EDG2 (extracellular) Blocking Peptide (#BLP-LR031) is the original antigen used for immunization during Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031) generation. The blocking peptide binds and ‘blocks’ Anti-LPAR1/EDG2 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain membrane (lanes 1 and 3), mouse brain membrane (lanes 2 and 4) and human brain glioblastoma U87 MG cell line (lanes 5 and 6) lysate:1,2,5. Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031), (1:400).
3,4,6. Anti-LPAR1 (EDG2) (extracellular) Antibody, preincubated with LPAR1/EDG2 (extracellular) Blocking Peptide (#BLP-LR031).
Expression of LPAR1 in rat corpus callosum (CC).Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LPAR1 (EDG2) (extracellular) Antibody (#ALR-031), (1:1000), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. LPAR1 immunoreactivity (red) appears in CC cells with oligodendrocyte morphology (arrows). B. Pre-incubation of the antibody with LPAR1 (EDG2) (extracellular) Blocking Peptide (#BLP-LR031), suppressed staining. Cell nuclei are stained with DAPI (blue).
Properties
Sequence
- (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor (Accession P6179).
Accession (Uniprot) Number P6179
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Concentration After Reconstitution 0.4 mg/ml.
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
