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LY 294002

A Specific Inhibitor of PI3-Kinase

Cat #: L-300
Lyophilized Powder yes
  • Bioassay Tested
  • Source Synthetic
    MW: 307.35
    Purity: >99%
    Effective concentration 5-10 µM.
    Structure
    Chemical name 2-(4-Morpholinyl)-8-phenyl-4H-1-benzopyran-4-one hydrochloride.
    Molecular formula C19H17NO3.
    CAS No.: 154447-36-6
    Activity LY 294002 is a potent and specific cell-permeable inhibitor of Pl3-kinase1.
    References-Activity
    1. Vlahos, C.J. et al. (1994) J. Biol. Chem. 269, 5241.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs LY 294002 inhibits AKT phosphorylation via PI3-kinase in NIH-3T3 cells.
      Alomone Labs LY 294002 inhibits AKT phosphorylation via PI3-kinase in NIH-3T3 cells.
      Cells were preincubated for 30 min in different concentrations of LY 294002 (#L-300) or Wortmannin (#W-400) and then stimulated with PDGF (3 ng/ml) for 20 min. Cell proteins were resolved by SDS-PAGE and probed with (A) anti-phospho-AKT antibody, followed by stripping and reprobed with (B) anti-Akt antibody.
    References - Scientific background
    1. Kapeller, R. et al. (1994) Bioessays 16, 565.
    2. Duronio, V. et al. (1998) Cell Signal10, 233.
    3. Vlahos, C.J. et al. (1994) J. Biol. Chem269, 5241.
    4. Vlahos, C.J. et al. (1995) J. Immunol154, 2413.
    5. Curnock, A.P. et al. (1998) Cell. Immunol187, 77.
    6. Nicholson, K.M. et al. (2003) Cancer Lett190, 31.
    7. Coffer, P.J. et al. (1998) Biochem. J335 (Pt 1), 1.
    8. Shao, J. et al. (2004) Cancer Res64, 229.
    9. Vivanco, I. et al. (2002) Nat. Rev. Cancer 2, 489.
    Scientific background

    PI 3-kinase is an enzyme implicated in growth factor signal transduction by associating with receptor and non-receptor tyrosine kinases.1,2 LY-294002 is a potent and specific cell-permeable inhibitor of Pl 3-kinase that acts on the ATP-binding site of the enzyme.3

    In in vitro assays it displays an IC50 of 1.4 µM on purified PI 3-kinase, but did not inhibit PKC, PKA, MAP kinase and PI 4-kinase.3 In fMet-Leu-Phe stimulated neutrophils, LY-294002 completely abolished PI 3-kinase activity at 50 µM.4 LY-294002 induces apoptosis in many cell types by blocking the PI3-K/Akt anti-apoptotic pathway.5,6 Thus LY-294002 is useful tool for identifying the biochemical events regulated by the PI3-K/Akt pathway.7 This pathway has important relevance to the cellular events involved in cancer.6,8,9

    Last update: 11/04/2021

    LY 294002 (#L-300) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Product citations
    1. Puckett, M.C. et al. (2013) Mol. Cell. Biol. 33, 2252.
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