Lys-Bradykinin

Kallidin, lysyl-bradykinin
A Ligand of B2 Bradykinin G-Protein Coupled Receptor
    Cat #: GPK-100
    Alternative Name Kallidin, lysyl-bradykinin
  • Lyophilized Powder
  • Bioassay Tested
  • Origin Synthetic peptide
    MW: 1188.39 Da.
    Purity: >98% (HPLC)
    Effective concentration 100 nM - 1 μM.
    Sequence KRPPGFSPFR.
    Molecular formula C56H85N17O12.
    CAS No.: 342-10-9.
    Activity Lys-Bradykinin is an endogenous nonspecific agonist for the B1 and B2 Bradykinin receptors, with some specificity towards the B2 receptor1. Activation of Bradykinin receptors with Lys-Bradykinin may induce intracellular Ca2+ increase1.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to one week at 4°C or three months at -20°C.
      • Lys-Bradykinin
        Alomone Labs Lys-Bradykinin activates dose dependently Ca2+ transients in rat aortic endothelium cells (RAEC).
        Cells were loaded with Fluo-3 AM and stimulated with increasing doses (0-400 nM) of Lys-Bradykinin (#GPK-100). A. Fluorescence levels before and after application (indicated by arrow) of control (black, saline perfusion) and 100 nM  Lys-Bradykinin (green). B. Normalized fluorescence plotted against Lys-Bradykinin concentrations.
    References - Scientific background
      • Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function1.

        Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct G protein-coupled receptors (GPCR): the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2)2.

        Activation of BKRB2 liberates mediators of vascular tone, fibrinolysis, and pain. BKRB2, which mediates most of the physiological effects of kinins, as well as BKRB1, represent potential therapeutic targets for treatment of inflammatory disorders and cardiovascular diseases.

        Expression of BKRB1 is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. A low level of expression of BKRB1 in the CNS of rodent and primates was recently demonstrated3. BKRB2 is constitutively and widely expressed throughout the CNS and peripheral nervous system and on various cell types including endothelial cells, nerve fibers, leukocytes, and mast cells3-5.

        Lys-Bradykinin is an endogenous nonspecific agonist for BKRB1and BKRB2, with some specificity towards the BKRB26. Activation of Bradykinin receptors with Lys-Bradykinin may induce intracellular Ca2+ elevation7. 100 nM Lys-Bradykinin induced Ca2+ transients in rat aortic endothelium cells (RAEC) (see figure here and review in reference #8).

    Target B2 bradykinin receptor
    Net Peptide Content: 100%
    Last update: 06/11/2019

    Lys-Bradykinin (GPK-100) is a highly pure, synthetic, and biologically active protein.

    For research purposes only, not for human use