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Certificate of Analysis

Lysophospholipid (LPA) Receptor Antibody Explorer Kit

A Screening Package of Lysophospholipid (LPA) Receptor Antibodies Economically Priced

Cat #: AK-515
Size: 6 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-LPAR1 (EDG2) (extracellular) Antibody Cat #: ALR-031

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor (Accession P61794). Extracellular, N-terminus.
Homology
Mouse – identical; human – 14/15 amino acid residues identical.

Anti-LPAR2 (EDG4) (extracellular) Antibody Cat #: ALR-032

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)NSGKELSLHWRPKD, corresponding to amino acid residues 16-29 of mouse LPA2 receptor (Accession Q9JL06). Extracellular, N-terminus.
Homology
Rat - identical; human - 13/14 amino acid residues identical.

Anti-LPAR3 (EDG7) (extracellular) Antibody Cat #: ALR-033

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide-(C)RSNTDTADEWTGTK, corresponding to amino acid residues 16-29 of rat LPA3 receptor (Accession Q8K5E0). Extracellular, N-terminus.
Homology
Mouse - identical; human - 12/14 amino acid residues identical.

Anti-LPAR4 (P2Y9) (extracellular) Antibody Cat #: ALR-034

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)DRRFIDFQFQDLNS, corresponding to amino acid residues 3-16 of mouse LPA4 receptor (Accession Q8BLG2).
Homology
Rat - identical, human - 13/14 amino acid residues identical.

Anti-Human LPAR5 (extracellular) Antibody Cat #: ALR-035

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H
Immunogen
Peptide (C)RSKLVAASVPARDRVR, corresponding to amino acid residues 261-276 of human LPA5 receptor (Accession Q9H1C0). 3rd extracellular loop.
Homology
Mouse, Rat – not recommended for use with mouse and rat samples.

Anti-LPAR6 (P2RY5) (extracellular) Antibody Cat #: ALR-036

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide STHSQGNNASEAC, corresponding to amino acid residues 156-168 of human LPA receptor 6 (Accession P43657). 2nd extracellular loop.
Homology
Rat, mouse -12/13 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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