MCU Blocking Peptide (#BLP-CC328) is the original antigen used for immunization during Anti-MCU Antibody (#ACC-328) generation. The blocking peptide binds and ‘blocks’ Anti-MCU primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat kidney (lanes 1 and 5), rat skeletal muscle (lanes 2 and 6), rat brain (lanes 3 and 7) and mouse brain (lanes 4 and 8) lysates:
- Expression of MCU in mouse midbrain.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-MCU Antibody (#ACC-328), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse substantia nigra region, showed immunoreactivity (green) in neuronal profiles in the substantia nigra pars compacta (SNC, horizontal arrows). B. Pre-incubation of the antibody with MCU Blocking Peptide (BLP-CC328), suppressed staining. Cell nuclei are stained with DAPI (blue). SNR = substantia nigra pars reticulata.
- CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter (Accession Q8NE86).