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Certificate of Analysis

Mechanosensitive Ion Channel Antibody Explorer Kit

A Screening Package of Mechanosensitive Ion Channel Antibodies Economically Priced

Cat #: AK-234
Size: 32 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-ASIC1 Antibody Cat #: ASC-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
Homology
Human - identical; mouse - 19/20 amino acid residues identical.

ASIC1 Blocking Peptide Cat #: BLP-SC014

Size: 40 µg

Anti-ASIC2a Antibody Cat #: ASC-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a (Accession Q16515). Intracellular, N-terminus.
Homology
Rat, mouse - identical.

ASIC2a Blocking Peptide Cat #: BLP-SC012

Size: 40 µg

Anti-ASIC3 Antibody Cat #: ASC-018

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide KPRSGLEEAQRRQAS(C), corresponding to amino acid residues 2-16 of rat ASIC3 (Accession O35240). Intracellular, N-terminus.
Homology
Mouse - 14/15 amino acid residues identical; human - 11/15 amino acid residues identical.

ASIC3 Blocking Peptide Cat #: BLP-SC018

Size: 40 µg

Anti-ENaC α (SCNN1A) (extracellular) Antibody Cat #: ASC-030

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)EELDRITEQTLFD, corresponding to amino acid residues 173-185 of rat ENaCα (Accession P37089). Extracellular loop.
Homology
Mouse, human - identical.

ENaC α/SCNN1A (extracellular) Blocking Peptide Cat #: BLP-SC030

Size: 40 µg

Anti-ENaC β (SCNN1B) (extracellular) Antibody Cat #: ASC-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: M, R
Immunogen
Peptide EFNYRTIEESPANNI(C), corresponding to amino acid residues 498-513 of rat ENaCβ (Accession P37090). Extracellular.
Homology
Mouse - identical; human - 14/15 amino acid residues identical.

ENaC β/SCNN1B (extracellular) Blocking Peptide Cat #: BLP-SC019

Size: 40 µg

Anti-ENaC γ (SCNN1G) (extracellular) Antibody Cat #: ASC-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IH, WB Reactivity: R
Immunogen
Peptide (C)YGVKESRKRREAGS, corresponding to amino acid residues 129-142 of rat ENaCγ (Accession P37091). Extracellular.
Homology
Human - 13/17 amino acid residues identical.

ENaC gamma/SCNN1G (extracellular) Blocking Peptide Cat #: BLP-SC011

Size: 40 µg

Anti-KCNK2 (TREK-1) Antibody Cat #: APC-047

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2 (Accession O95069). Intracellular, N-terminus.
Homology
Rat - identical; mouse - 17/18 amino acid residues identical.

KCNK2/TREK-1 Blocking Peptide Cat #: BLP-PC047

Size: 40 µg

Anti-KCNK4 (TRAAK) Antibody Cat #: APC-108

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4 (Accession Q9NYG8). Intracellular, C-terminus.
Homology
Rat, mouse, dog - identical.

KCNK4/TRAAK Blocking Peptide Cat #: BLP-PC108

Size: 40 µg

Anti-KCNK10 (TREK-2) Antibody Cat #: APC-055

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, R
Immunogen
Peptide TFRNYSLDEEKKEDETEKMC, corresponding to amino acid residues 475-494 of rat KCNK10 (Accession Q9JIS4). Intracellular, C-terminal domain.
Homology
Human - 19/20 amino acid residues identical.

KCNK10/TREK-2 Blocking Peptide Cat #: BLP-PC055

Size: 40 µg

Anti-KCNK18 (TRESK) (extracellular) Antibody Cat #: APC-122

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18 (Accession Q6VV64). 1st extracellular loop.
Homology
Rat - 13/14 amino acid residues identical.

KCNK18/TRESK (extracellular) Blocking Peptide Cat #: BLP-PC122

Size: 40 µg

Anti-KCNQ4 Antibody Cat #: APC-164

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)DRKTREKGDKGPSD, corresponding to amino acid residues 594-607 of mouse KCNQ4 (Accession Q9JK97). Intracellular, C-terminus.
Homology
Rat - identical; human - 12/14 amino acid residues identical.

KCNQ4 Blocking Peptide Cat #: BLP-PC164

Size: 40 µg

Anti-TRPA1 (extracellular) Antibody Cat #: ACC-037

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)NSTGIINETSDHSE, corresponding to amino acid residues 747-760 of human TRPA1 (Accession O75762). 1st extracellular loop.
Homology
Bovine - identical; canis - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical; mouse - 9/14 amino acid residues identical.

TRPA1 (extracellular) Blocking Peptide Cat #: BLP-CC037

Size: 40 µg

Anti-TRPC1 Antibody Cat #: ACC-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide QLYDKGYTSKEQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995). Intracellular.
Homology
Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus Laevis - 11/14 amino acid residues identical.

TRPC1 Blocking Peptide Cat #: BLP-CC010

Size: 40 µg

Anti-TRPC6 Antibody Cat #: ACC-017

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide (C)RRNESQDYLLMDELG, corresponding to amino acid residues 24-38 of mouse TRPC6 (Accession Q61143). Intracellular, N-terminus.
Homology
Rat - identical; human - 15/16 amino acid residues identical.

TRPC6 Blocking Peptide Cat #: BLP-CC017

Size: 40 µg

Anti-TRPP1 (PKD2) Antibody Cat #: ACC-052

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of human TRPP1 (Accession Q13563). Intracellular, C-terminus.
Homology
Mouse - 12/14 amino acid residues identical; rat - 11/14 amino acid residues identical.

TRPP1/PKD2 Blocking Peptide Cat #: BLP-CC052

Size: 40 µg

Anti-TRPV4 Antibody Cat #: ACC-034

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CDGHQQGYAPKWRAEDAPL, corresponding to amino acid residues 853-871 of rat TRPV4 (Accession Q9ERZ8). Intracellular, C-terminus.
Homology
Mouse - 17/19 amino acid residues identical; human - 16/19 amino acid residues identical.

TRPV4 Blocking Peptide Cat #: BLP-CC034

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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