mGluR7 (extracellular) Blocking Peptide (#BLP-GC017) is the original antigen used for immunization during Anti-mGluR7 (extracellular) Antibody (#AGC-017) generation. The blocking peptide binds and ‘blocks’ Anti-mGluR7 (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse (lanes 1 and 4) and rat (lanes 2 and 5) brain membranes and human CCF-STTGI astrocytoma (lanes 3 and 6) cell line lysate (1:200):
- Expression of mGluR7 in rat neocortexImmunohistochemical staining of rat neocortex frozen sections using Anti-mGluR7 (extracellular) Antibody (#AGC-017). A. mGluR7 staining (green) appears in several neocortical neurons. B. Merge image showing mGluR7 staining together with cell nuclei (blue).
- TISGSKKEDTDRKC, corresponding to amino acid residues 377-390 of rat mGluR7 (Accession P35400).