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Certificate of Analysis

Microglia GPCR Antibody Explorer Kit

A Screening Package of Antibodies to GPCRs Expressed in Microglia Economically Priced

Cat #: AK-630
Size: 24 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody Cat #: AOR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CSPAPGSWLNLSHVDGN, corresponding to amino acid residues 22-38 of the rat µ-Opioid receptor (Accession P33535). Extracellular, N-terminus.
Homology
Mouse - identical; human - 15/17 amino acid residues identical.

µ-Opioid Receptor/OPRM1 (extracellular) Blocking Peptide Cat #: BLP-OR011

Size: 40 µg

Anti-Adenosine A2A Receptor (extracellular) Antibody Cat #: AAR-008

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)SQKDENSTKT(S)GE, corresponding to amino acid residues 143-156 of mouse adenosine A2A receptor (Accession Q60613). 2nd extracellular loop.
Homology
Mouse – 13/14 amino acid residues identical; rat – 12/14 amino acid residues identical.

Adenosine A2A Receptor (extracellular) Blocking Peptide Cat #: BLP-AR008

Size: 40 µg

Anti-Human Adenosine A2A Receptor (extracellular) Antibody Cat #: AAR-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: H
Immunogen
Peptide CGQPKEGKNHSQG, corresponding to amino acid residues 146 - 158 of human adenosine A2A receptor (Accession P29274). 2nd extracellular loop.
Homology
Human only.

Human Adenosine A2A Receptor (extracellular) Blocking Peptide Cat #: BLP-AR007

Size: 40 µg

Anti-EMR1 (ADGRE1) (extracellular) Antibody Cat #: AER-051

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.
Homology
Mouse, Rat –11/14 amino acid residues identical.

EMR1/ADGRE1 (extracellular) Blocking Peptide Cat #: BLP-ER051

Size: 40 µg

Anti-Human FPR2/ALX (extracellular) Antibody Cat #: AFR-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H
Immunogen
Peptide (C)GGTPEERLKVAIT, corresponding to amino acid residues 184-196 of human FPR2/ALX (Accession P25090). 2nd extracellular loop.
Homology
Human only.

Human FPR2/ALX (extracellular) Blocking Peptide Cat #: BLP-FR002

Size: 40 µg

Anti-GPR84 (extracellular) Antibody Cat #: AGR-052

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84 (Accession Q9NQS5). 2nd extracellular loop.
Homology
Human – 14/15 amino acid residues identical; mouse, rat – 13/15 amino acid residues identical.

GPR84 (extracellular) Blocking Peptide Cat #: BLP-GR052

Size: 40 µg

Anti-LPAR6 (P2RY5) (extracellular) Antibody Cat #: ALR-036

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IFC, LCI, WB Reactivity: H, M, R
Immunogen
Peptide STHSQGNNASEAC, corresponding to amino acid residues 156-168 of human LPA receptor 6 (Accession P43657). 2nd extracellular loop.
Homology
Rat, mouse -12/13 amino acid residues identical.

LPAR6/P2RY5 (extracellular) Blocking Peptide Cat #: BLP-LR036

Size: 40 µg

Anti-mGluR5 (extracellular) Antibody Cat #: AGC-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide EGFAQENSKYNKTC, corresponding to amino acids 367-380 of rat mGluR5 (Accession P31424). Extracellular, N-terminus.
Homology
Mouse - identical; human - 13/14 amino residues identical.

mGluR5 (extracellular) Blocking Peptide Cat #: BLP-GC007

Size: 40 µg

Anti-P2Y2 Receptor (extracellular) Antibody Cat #: APR-102

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2 (Accession P41232). Extracellular, C-terminus.
Homology
Rat, mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.

P2Y2 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR102

Size: 40 µg

Anti-P2Y6 Receptor Antibody Cat #: APR-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, WB Reactivity: H, M, R
Immunogen
Peptide CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession Q63371). Intracellular, C-terminus.
Homology
Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.

P2Y6 Receptor Blocking Peptide Cat #: BLP-PR011

Size: 40 µg

Anti-P2Y12 Receptor (extracellular) Antibody Cat #: APR-020

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, LCI, WB Reactivity: H, R
Immunogen
Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.
Homology
Rat, mouse - 12/13 amino acid residues identical.

P2Y12 Receptor (extracellular) Blocking Peptide Cat #: BLP-PR020

Size: 40 µg

Anti-P2Y13 Receptor Antibody Cat #: APR-017

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, WB Reactivity: H, M, R
Immunogen
Peptide DRFLKIIRPLRNIFLK(C), corresponding to amino acid residues 119-134 of human P2RY13 (Accession Q9BPV8). 2nd intracellular loop.
Homology
Mouse - 11/16 amino acid residues identical; rat - 10/16 amino acid residues identical.

P2Y13 Receptor Blocking Peptide Cat #: BLP-PR017

Size: 40 µg

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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