Certificate of Analysis

Mouse NGF Deluxe Research Pack

All You Need for NGF Research

Cat #: ESD-651
Size: 10 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-NGF Antibody Cat #: AN-240

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Highly purified 2.5 S mouse NGF (Accession P01139).
Homology
Rat- 115/120 amino acid residues identical; human- 107/120 amino acid residues identical.

Mouse NGF/proNGF Neutralizing Antibody Cat #: ALM-006

Size: 0.25 mg Host: Mouse Type: Monoclonal Application: IE, N Reactivity: H, M
Immunogen
Highly purified 2.5S mouse NGF (Accession P01139).

Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.

Anti-p75 NGF Receptor (extracellular)-ATTO Fluor-488 Antibody Cat #: ANT-007-AG

Size: 50 µl Host: Rabbit Type: Polyclonal Application: FC, IC, IF, IH, LCI Reactivity: H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.
Label
ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Anti-TrkA (extracellular) Antibody Cat #: ANT-018

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739). 2nd extracellular immunoglobulin-like domain.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody Cat #: ANT-018-FR

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH Reactivity: H, M, R
Immunogen
Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739). Extracellular, 2nd immunoglobulin-like domain.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.
Label
ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.

Pharmacological Reagents

K252a Cat #: K-150

Size: 0.1 mg Source: Natural MW: 467.5 Purity: >98%
Origin
Nocardiopsis sp. (soil fungi).
Effective concentration
10-200 nM.
Activity
K252a inhibits a variety of kinases including PKC (IC50 = 25 nM), PKA (IC50 = 18 nM) and PKG (IC50 = 20 nM)1. It also inhibits CaMK (IC50 = 1.8 nM) and MLCK (IC50 = 17 nM). K252a has also been shown to inhibit NGF mediated proliferation through the Trk receptor family2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
DMSO. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to one week at 4°C or three months at -20°C.
Protect from light.

GW441756 hydrochloride Cat #: G-191

Size: 25 mg Source: Synthetic MW: 311.8 Purity: ≥97%
Effective concentration
2 nM - 2 µM.
Activity
GW441756 is a potent, selective inhibitor of the NGF receptor tyrosine kinase A (TrkA)1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
4-10 mM in H2O. Centrifuge all product preparations before use (5,000 x g for 1 min).
Storage after reconstitution
Up to four weeks at 4°C or three months at -20°C.

PACA Cat #: P-340

Size: 10 mg Source: Synthetic MW: 217.22 Purity: >98%
Effective concentration
5-20 µM
Activity
N-propargyl caffeate amide (PACA) potentiates 2 ng/ml NGF-induced neurite outgrowth in PC12 cells at 5-20 µM. PACA was also shown to reduce 6-OHDA-induced neurotoxicity in dopaminergic PC12 cells and primary rat midbrain neurons, possibly by activating the Nrf2/HO-1 pathway1.
Storage before reconstitution
Store at -20°C.
Reconstitution
Soluble in DMSO up to 100 mM. Spin down all products before handling.
Storage after reconstitution
Store at -20°C. It is recommended to aliquot stock solutions to avoid repeated thawing and freezing.

Native mouse NGF 2.5S protein (>95%) Cat #: N-100

Size: 0.1 mg MW: 26 kDa Purity: >95% (HPLC) Net peptide content: 100%
Origin
Native protein isolated from mouse submaxillary glands.
Effective concentration
EC50 = 0.7 nM.
Activity
NGF is purified in three forms: the 7S, 2.5S and β. The 2.5S form is 9 amino acids shorter than the β form because of proteolysis that occurs during the purification process1. NGF has been shown to regulate neuronal survival, development, function and plasticity2. The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water, DMEM at a concentration of at least 10 μg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note
A mouse monoclonal antibody (#ALM-006) is included in this Research Pack. Please take into account when reacting with a secondary antibody.
For research purposes only, not for human use
Shipping and Ordering information