Name: Mouse proBDNF Basic Research Pack
Brand: Alomone Labs
SKU: ESB-602

Cat #: ESB-602

Size: 6 Vials

Form: Lyophilized

Antibodies

Anti-proBDNF Antibody Cat #: ANT-006

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IF, IH, IP, WB Reactivity: H, M, R

Immunogen

Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Pro-domain of the BDNF protein.

Homology

Mouse, rat - 16/17 amino acid residues identical.

proBDNF Blocking Peptide Cat #: BLP-NT006

Size: 40 µg

Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R

Immunogen

Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75^NTR (Accession P08138). Extracellular, stalk domain.

Homology

Mouse - identical; rat - 15/16 amino acid residues identical.

p75 NGF Receptor (extracellular) Blocking Peptide Cat #: BLP-NT007

Size: 40 µg

Pharmacological Reagents

Recombinant mouse proBDNF protein Cat #: B-240

Size: 25 µg MW: 52.4 kDa Purity: >98% (HPLC) Net peptide content: 100%

Origin

Recombinant, E. coli

Effective concentration

0.2 nM.

Activity

BDNF is a neurotrophic factor and binds p75^NTR as well as TrkB receptors^1,2. BDNF supports the survival of many cell types^3-8. proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons⁹ expressing both p75 and sortilin, and to be involved in LTD¹⁰.

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Sterile water at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage after reconstitution

Up to one week at 4°C or four-six weeks at -70°C.

Recombinant mouse proBDNF (cleavage resistant) protein Cat #: B-243

Size: 25 µg MW: 52 kDa Purity: >98% (HPLC) Net peptide content: 100%

Origin

Recombinant, E. coli

Effective concentration

0.2 nM.

Activity

Storage before reconstitution

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Sterile water at a concentration of at least 10 µg/ml.
Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.

Storage after reconstitution

Up to one week at 4°C or four-six weeks at -70°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use

Shipping and Ordering information

Order Summary

Certificate of Analysis

Mouse proBDNF Basic Research Pack