Certificate of Analysis

Mouse proNGF Deluxe Research Pack

All You Need for proNGF Research
Cat #: ESD-653 11 Vials

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-proNGF Antibody Cat #: ANT-005

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor) (Accession P25427). Pro-domain of the NGF protein.
Homology
Mouse - identical; human - 16/21 amino acid residues identical.

Guinea pig Anti-proNGF Antibody Cat #: AGP-031

Size: 0.2 ml Host: Guinea pig Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor) (Accession P25427). Pro-domain of the NGF protein.
Homology
Mouse - identical; human - 16/21 amino acid residues identical.

Anti-p75 NGF Receptor (extracellular) Antibody Cat #: ANT-007

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, IP, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.

Anti-p75 NGF Receptor (extracellular)-ATTO-488 Antibody Cat #: ANT-007-AG

Size: 50 µl Host: Rabbit Type: Polyclonal Application: FC, IC, IF, IH, LCI Reactivity: H, M, R
Immunogen
Peptide CEEIPGRWITRSTPPE corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.
Homology
Mouse - identical; rat - 15/16 amino acid residues identical.
Label
ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.

Mouse NGF/proNGF Neutralizing Antibody Cat #: ALM-006

Size: 0.25 mg Host: Mouse Type: Monoclonal Application: IE, N Reactivity: H, M
Immunogen
Highly purified 2.5S mouse NGF (Accession P01139).

Pharmacological Reagents

Recombinant mouse proNGF protein Cat #: N-250

Size: 25 µg MW: 50 kDa. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
0.4 - 4 nM.
Activity
proNGF is the preferred ligand of p75NTR 1. In contrast to its mature form proNGF induces apoptosis via p75NTR 1, but can also mediate cell survival in DRGs2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water, DMEM at a concentration of at least 10 µg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Recombinant mouse proNGF (cleavage resistant) protein Cat #: N-255

Size: 5 µg MW: 50 kDa. Purity: >98% (HPLC) Net peptide content: 100%
Origin
Recombinant, E. coli
Effective concentration
0.4 - 4 nM.
Activity
proNGF is the preferred ligand of p75NTR 1. In contrast to its mature form proNGF induces apoptosis via p75NTR 1, but can also mediate cell survival in DRGs2 and in superior cervical ganglion (SCG)3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Sterile water, DMEM at a concentration of at least 10 μg/ml. Centrifuge all product preparations before use (10000 x g 5 min). Repeated freezing/thawing might result in loss of activity.
Storage after reconstitution
Up to one week at 4°C or four-six weeks at -70°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

Note
A guinea pig polyclonal antibody (#AGP-031) and mouse monoclonal antibody (#ALM-006) are included in this Research Pack. Please take into account when reacting with a secondary antibody.