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Muscarinic Toxin 3

MT3, MT-3

An Antagonist of M1 and M4 Muscarinic Receptors and Adrenoceptors

Cat #: M-140
Alternative Name MT3, MT-3
Lyophilized Powder yes
  • Bioassay Tested
  • Origin Natural protein isolated from Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
    MW: 7379 Da
    Purity: >99% (HPLC)
    Form Lyophilized powder.
    Effective concentration 100-1000 nM.
    Modifications Disulfide bonds between Cys3-Cys24, Cys17-Cys42, Cys46-Cys57and Cys58-Cys63.
    Molecular formula C319H489N89O97S8.
    Activity Muscarinic receptor 4 and adrenoceptor antagonist1-4.
    1. Jolkkonen, M. et al. (1994) FEBS Lett. 352, 91.
    2. Karlsson, E. et al. (1994) Ann. N.Y. Acad. Sci. 710, 153.
    3. Caulfield, M.P. and Birdsall, N.J. (1998) Pharm. Rev. 50, 279.
    4. Nareoja, K. et al. (2011) Br. J. Pharmacol. 164, 538.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Muscarinic Toxin 3 inhibits Muscarinic Receptor 4 expressed in CHO-K1 cells.
      Alomone Labs Muscarinic Toxin 3 inhibits Muscarinic Receptor 4 expressed in CHO-K1 cells.
      CHO-K1 cells were co-transfected with human M4 and Gα15 in order to couple the M4 receptor to the Ca2+ signaling pathway. Ca2+ concentrations were monitored using FLIPR. Cells were first incubated in the presence of different concentrations of Muscarinic Toxin 3 (#M-140) followed by Oxotremorine. The percentage of M4 receptor inhibition was plotted versus different concentrations of Muscarinic Toxin 3. The IC50 value of Muscarinic Toxin 3 was between 100 to 1000 nM.
    References - Scientific background
    1. Bradley, K.N. (2000) Pharmacol. Ther. 85, 87.
    2. Nareoja, K. et al. (2011) Br. J. Pharmacol. 164, 538.
    Scientific background Muscarinic toxins are isolated from the venom of the mamba species and are composed of 65 to 66 amino acid residues with four intramolecular disulfide bridges. One of these is muscarinic toxin 3 (MT-3) which was isolated from the green mamba (Dendroaspis augusticeps) and is composed of 65 amino acid residues1. A recent study investigated the interaction of MT3 with cloned receptors (mAChRs and adrenoceptors) expressed in insect cells by radioligand binding. MT3 appears to have a broad spectrum of targets showing high-affinity binding (IC50 = 1-10 nM) to M4 mAChR, α1A-, α1D- and α2A-adrenoceptors and lower affinity binding (IC50 > 25 nM) to α1B- and α2C-adrenoceptors and M1 mAChR2.
    Target M1, M4 muscarinic receptors, adrenoceptors
    Peptide Content: 100%
    Last update: 06/11/2022

    Muscarinic Toxin 3 (#M-140) is a highly pure, natural, and biologically active peptide toxin.

    For research purposes only, not for human use
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