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Certificate of Analysis

Muscle-Type nAChR Deluxe Research Pack

All You Need for nAChR Research

Cat #: ESD-900
Size: 8 Vials
Form: Lyophilized

Antibodies

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody Cat #: ANC-001

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide EHETRLVAKLFKD(C), corresponding to amino acid residues 22-34 of rat nAChRα1 (Accession P25108). Extracellular, N-terminus.
Homology
Human - identical; mouse - 12/13 amino acid residues identical.

Nicotinic Acetylcholine Receptor α1/CHRNA1 (extracellular) Blocking Peptide Cat #: BLP-NC001

Size: 40 µg

Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody Cat #: ANC-011

Size: 0.2 ml Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)EKDEEMSTKVYLD, corresponding to amino acid residues 68-80 of rat nAChRβ1 (Accession P25109). Extracellular, N-terminus.
Homology
Mouse, human - identical.

Nicotinic Acetylcholine Receptor β1/CHRNB1 (extracellular) Blocking Peptide Cat #: BLP-NC011

Size: 40 µg

Pharmacological Reagents

αA-Conotoxin PIVA Cat #: STC-600

Size: 0.1 mg MW: 2651 Da Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-20 nM
Activity
αA-Conotoxin PIVA reversibly blocks muscle α1/β1/γ/δ nicotinic ACh receptor1,2.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

αC-Conotoxin PrXA Cat #: STC-620

Size: 0.1 mg MW: 3541 Da Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
1-20 nM.
Activity
αC-Conotoxin PrXA reversibly blocks both adult and fetal mammalian muscle α1/β1/ε/δ and α1/β1/γ/δ nicotinic ACh receptor1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Azemiopsin Cat #: STA-100

Size: 0.5 mg MW: 2544 Da Purity: >98% (HPLC) Net peptide content: 100%
Origin
Synthetic peptide
Effective concentration
0.5-10 µM.
Activity
Azemiopsin blocks the adult form (α1/β1/ε/δ) as well as the fetal form (α1/β1/γ/δ) nAChR1.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any other aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

α-Bungarotoxin-Biotin Cat #: B-100-B

Size: 10 µg MW: ~9030 Da Purity: >95% (HPLC) Net peptide content: 100%
Origin
Modified natural protein isolated from Bungarus multicinctus (Many-banded krait).
Effective concentration
100-200 nM
Activity
α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs), thereby preventing the depolarizing action on postsynaptic membranes and blocking neuromuscular transmission. Selective for α7 receptors (IC50 value of 1.6 nM) and α3/β4 receptors (IC50 value of >3 μM)1,2. The toxin also blocks GABA(A) receptor subtypes3.
Storage before reconstitution
Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution
Any aqueous buffer. Centrifuge all product preparations before use (10000 x g 5 min).
Storage after reconstitution
Up to two weeks at 4°C or three months at -20°C.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For more information, please refer to individual certificate of analysis for each product.

For research purposes only, not for human use
Shipping and Ordering information

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