N-Cadherin Blocking Peptide (#BLP-NR082) is the original antigen used for immunization during Anti-N-Cadherin Antibody (#ANR-082) generation. The blocking peptide binds and ‘blocks’ Anti-N-Cadherin primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of mouse brain (lanes 1 and 5), mouse heart (lanes 2 and 6), rat heart (lanes 3 and 7) and human brain neuroblastoma (SH-SY5Y) (lanes 4 and 8) cell lysates:
- Expression of N-Cadherin in mouse cortexImmunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-N-Cadherin Antibody (#ANR-082), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. N-Cadherin immunoreactivity (green) appears in apical dendrites (horizontal arrows) and in the soma (vertical arrows) of pyramidal neurons. B. Pre-incubation of the antibody with N-Cadherin Blocking Peptide (BLP-NR082), suppressed staining. Cell nuclei are stained with DAPI (blue).
- (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2 (Accession P15116).