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Certificate of Analysis

nAChR Antibodies for Pain Research Explorer Kit

A Screening Package of nAChR Antibodies for Pain Research Economically Priced

Cat #: AK-375
Size: 4 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody Cat #: ANC-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRα6 (Accession P43143). Extracellular, N-terminus.
Homology
Mouse - 12/13 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody Cat #: ANC-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)KELVKNYNPLER, corresponding to amino acid residues 31-42 of rat nAChRα7 (Accession Q05941). Extracellular, N-terminus.
Homology
Mouse, human - identical.

Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody Cat #: ANC-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KDHKATNSKGSEWKK, corresponding to amino acid residues 436-450 of rat nAChRα9 (Accession P43144). 2nd intracellular loop.
Homology
Mouse, human – identical.

Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody Cat #: ANC-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRβ4 (Accession P12392). Extracellular, N-terminus.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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