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Certificate of Analysis

nAChR Antibody Explorer Kit

A Screening Package of nAChR Antibodies Economically Priced

Cat #: AK-203
Size: 13 Vials
Form: Lyophilized

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody Cat #: ANC-001

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide EHETRLVAKLFKD(C), corresponding to amino acid residues 22-34 of rat nAChRα1 (Accession P25108). Extracellular, N-terminus.
Homology
Human - identical; mouse - 12/13 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody Cat #: ANC-002

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DLEQMERTVDLKD, corresponding to amino acid residues 189-201 of rat nAChRα2 (Accession P12389). Extracellular, N-terminus.
Homology
Mouse - identical; human - 12/13 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody Cat #: ANC-003

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: M, R
Immunogen
Peptide (C)KWKPSDYQGVEFMR, corresponding to amino acid residues 91-104 of rat nAChRα3 (Accession P04757). Extracellular, N-terminus.
Homology
Mouse - identical; human - 11/14 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody Cat #: ANC-004

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)DLVNMHSRVDQLD, corresponding to amino acid residues 190-202 of human nAChRα4 (Accession P43681). Extracellular, N-terminus.
Homology
Rat, mouse - 12/13 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody Cat #: ANC-005

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)EGASTKTVVRYNGT, corresponding to amino acid residues 129-142 of rat nAChRα5 (Accession P20420). Extracellular, N-terminus.
Homology
Mouse - identical; human - 12/14 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor α6 (CHRNA6) (extracellular) Antibody Cat #: ANC-006

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)EQLFHTLFAHYNR, corresponding to amino acid residues 35-47 of rat nAChRα6 (Accession P43143). Extracellular, N-terminus.
Homology
Mouse - 12/13 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody Cat #: ANC-007

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IFC, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)KELVKNYNPLER, corresponding to amino acid residues 31-42 of rat nAChRα7 (Accession Q05941). Extracellular, N-terminus.
Homology
Mouse, human - identical.

Anti-Nicotinic Acetylcholine Receptor α9 (CHRNA9) Antibody Cat #: ANC-019

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IF, IH, WB Reactivity: H, M, R
Immunogen
Peptide (C)KDHKATNSKGSEWKK, corresponding to amino acid residues 436-450 of rat nAChRα9 (Accession P43144). 2nd intracellular loop.
Homology
Mouse, human – identical.

Anti-Nicotinic Acetylcholine Receptor α10 (CHRNA10) Antibody Cat #: ANC-010

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: M, R
Immunogen
Peptide (C)RRHEDWKRLARVMDR, corresponding to amino acid residues 411-425 of rat nAChRα10 (Accession Q9JLB5). 2nd intracellular loop.
Homology
Mouse – identical; human – 13/15 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor β1 (CHRNB1) (extracellular) Antibody Cat #: ANC-011

Size: 50 µl Host: Rabbit Type: Polyclonal Application: WB Reactivity: H, M, R
Immunogen
Peptide (C)EKDEEMSTKVYLD, corresponding to amino acid residues 68-80 of rat nAChRβ1 (Accession P25109). Extracellular, N-terminus.
Homology
Mouse, human - identical.

Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody Cat #: ANC-012

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide (C)EDFDNMKKVRLP, corresponding to amino acid residues 96-107 of rat nAChRβ2 (Accession P12390). Extracellular, N-terminus.
Homology
Mouse - identical; human - 11/12 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor β3 (CHRNB3) (extracellular) Antibody Cat #: ANC-013

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, LCI, WB Reactivity: M, R
Immunogen
Peptide (C)NAKGMKGNRREG, corresponding to amino acid residues 211-222 of rat nAChRβ3 (Accession P12391). Extracellular, N-terminus.
Homology
Mouse - identical; human - 11/12 amino acid residues identical.

Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody Cat #: ANC-014

Size: 50 µl Host: Rabbit Type: Polyclonal Application: IC, IF, IH, LCI, WB Reactivity: H, M, R
Immunogen
Peptide CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRβ4 (Accession P12392). Extracellular, N-terminus.
Homology
Mouse - identical; human - 13/14 amino acid residues identical.

Antibody Storage & Reconstitution Instructions

Storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Reconstitution

Add deionized water, depending on the sample size.

Storage after reconstitution

The reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C or below.
Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).

Control antigen storage before reconstitution

Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.

Control antigen reconstitution

100 μl water.

Control antigen storage after reconstitution

-20ºC.

Preadsorption control

1 μg peptide per 1 μg antibody.

For research purposes only, not for human use
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