Nav1.5/SCN5A (493-511) Blocking Peptide (#BLP-SC005) is the original antigen used for immunization during Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005) generation. The blocking peptide binds and ‘blocks’ Anti-Nav1.5/SCN5A (493-511) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
- Western blot analysis of rat heart membranes:
- Western blot analysis of NaV1.5 in NaV1.5 transfected HEK-293 cells:
- Expression of NaV1.5 in rat heartImmunohistochemical staining of NaV1.5 in rat myocardium paraffin-embedded sections using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:100). Staining is specific for cardiomyocytes while smooth muscles cells in the small vessels are negative (red arrows). Hematoxilin is used as the counterstain.
- Expression of NaV1.5 in mouse heartImmunohistochemical staining of mouse heart sections using Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005), (1:200). NaV1.5 staining (green) is part of the striate morphology. Nuclei are stained with DAPI (blue).
- Western blot analysis of human-NaV1.5 transfected HEK-293 cell lysate (lanes 1 and 3) and rat heart membrane protein (lanes 2 and 4):
- Expression of NaV1.5 in rat heartImmunohistochemical staining of rat heart frozen sections using Guinea pig Anti-NaV1.5 (SCN5A) Antibody (#ASC-005-GP). NaV1.5 staining (red) appears in cardiomyocytes and in intercalated discs (arrows). Nuclei are stained using DAPI (blue).
- Human heart sections (1:200) (Cheng, J. et al. (2017) Sci. Rep. 7, 42953.).
- DRLPKSDSEDGPRALNQLS(C), corresponding to amino acid residues 493-511 of rat NaV1.5 (Accession P15389).