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Certificate of Analysis

Nav1.7/SCN9A Blocking Peptide

Overview

Cat #: BLP-SC008
Size: 40 µg
Type: Synthetic peptide
Form: Lyophilized powder
Applications: bl

Application key:

BL- Blocking
For research purposes only, not for human use

Properties

Properties

Sequence
  • (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7  (Accession O08562).
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 μg peptide per 1 μg antibody.
Standard Quality Control Of Each Lot Western blot analysis.

Applications

Applications

Blocking
  • Western blot analysis of DRG lysates:
    Western blot analysis of DRG lysates:
    1. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
    2. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
  • Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):
    Western blot analysis of ND7/23 cell lysate (lanes 1,3) and rat brain membranes (lanes 2,4):
    1,2. Anti-NaV1.7 (SCN9A) Antibody (#ASC-008), (1:200).
    3,4. Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
  • Rat dorsal root ganglion (DRG) frozen section.

    Human cervical tissue (1:25) (Hernandez-Plata, E. et al. (2012) Int. J. Cancer 130, 2013.).

    Mouse dorsal root ganglia (DRGs) (1:1000) (Amaya, F. et al. (2006) J. Neurosci. 26, 12852.).
  • Rat dorsal root ganglion (DRG).
  • Expression of NaV1.7 in rat DRG
    Expression of NaV1.7 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR). A. NaV1.7 staining (purple) appears in DRG neurons. B. Nuclear staining using DAPI as the counterstain. C. Merge images of A and B.
  • Western blot analysis of rat DRG lysates:
    Western blot analysis of rat DRG lysates:

    1. Guinea pig Anti-NaV1.7 (SCN9A) Antibody (#AGP-057), (1:400).
    2. Guinea pig Anti-NaV1.7 (SCN9A) Antibody, preincubated with Nav1.7/SCN9A Blocking Peptide (#BLP-SC008).
     
    Following a broad screen of secondary antibodies, the following was used for this application:
    Western blot analysis:
    #106-035-006 (Jackson ImmunoResearch).
    Note: addition of 0.5% Tween-20 to the primary antibody solution may strengthen the signal and lower background.

Last update: 11/04/2021
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